InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Wnk1

Summary

InnateDB Protein

IDBP-183038.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Wnk1

Protein Name

WNK lysine deficient protein kinase 1

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000086017

InnateDB Gene

IDBG-183036 (Wnk1)

Protein Structure

UniProt Annotation

Function

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide- sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. {ECO:0000269PubMed:12671053}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:11498583}.

Disease Associations

Tissue Specificity

Widely expressed in both adult and embryonic tissue, with highest levels observed in the testis and lower levels in heart, lung, kidney, placenta, brain and skeletal muscle. Two isoforms are expressed in heart, a single shorter isoform in the kidney. Locates to the distal convoluted tubule, the medullary collecting duct and the cortical collecting duct of the kidney. HSN2-containing isoform 2 and isoform 3 are restricted to the nervous system, expressed preferentially in sensory neurons than in motor neurons and in general more abundant in axons than in cell bodies (at protein level). In the DRG, predominantly expressed in the satellite cells that envelop sensory neurons, but low expression also observed in the cell bodies of neurons (at protein level). In the sciatic nerve, expressed in the Schwann cells that surround axons and in a mosaic distribution of axons (at protein level). In the spinal cord, expressed in superficial layers (LI and LII), as well as in the fibers of the Lissauer tract (at protein level). Also detected in the axon fibers of dorsolateral funiculus and lateral funiculus (at protein level). {ECO:0000269PubMed:11498583, ECO:0000269PubMed:14514722, ECO:0000269PubMed:18521183}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 67 interaction(s) predicted by orthology.

Predicted by orthology
Total	67 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0004860	protein kinase inhibitor activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0019902	phosphatase binding

Biological Process

GO:0003084	positive regulation of systemic arterial blood pressure
GO:0006468	protein phosphorylation
GO:0006469	negative regulation of protein kinase activity
GO:0006811	ion transport
GO:0010923	negative regulation of phosphatase activity
GO:0035556	intracellular signal transduction
GO:0050794	regulation of cellular process
GO:0090188	negative regulation of pancreatic juice secretion

Cellular Component

GO:0005737

cytoplasm

Protein Structure and Domains

PDB ID

MGI:2442092

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain
IPR024678 Serine/threonine-protein kinase OSR1/WNK, CCT domain

PFAM

PF00069
PF07714
PF12202

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt