InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Uchl3

Summary

InnateDB Protein

IDBP-184384.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Uchl3

Protein Name

ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000002289

InnateDB Gene

IDBG-184380 (Uchl3)

Protein Structure

UniProt Annotation

Function

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome. {ECO:0000269PubMed:15884048, ECO:0000269PubMed:16816367, ECO:0000269PubMed:17460351, ECO:0000269PubMed:17967898, ECO:0000269PubMed:19837878, ECO:0000269PubMed:21762696}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:11341770, ECO:0000269PubMed:17967898}.

Disease Associations

Tissue Specificity

Ubiquitously expressed, with highest levels in brain, liver, heart, thymus, kidney and testis. Highly expressed in the cauda epididymidis, in meiotic pachytene spermatocytes and post-meiotic spematids. In the retina, enriched in the photoreceptor inner segment. {ECO:0000269PubMed:10713173, ECO:0000269PubMed:11341770, ECO:0000269PubMed:16816367, ECO:0000269PubMed:17460351}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.
They are also associated with 53 interaction(s) predicted by orthology.

Experimentally validated
Total	2 [view]
Protein-Protein	2 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	53 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004843	ubiquitin-specific protease activity
GO:0008233	peptidase activity

Biological Process

GO:0006511	ubiquitin-dependent protein catabolic process
GO:0007628	adult walking behavior
GO:0016579	protein deubiquitination
GO:0030163	protein catabolic process
GO:0032869	cellular response to insulin stimulus
GO:0042755	eating behavior
GO:0045600	positive regulation of fat cell differentiation
GO:0060041	retina development in camera-type eye

Cellular Component

GO:0005622	intracellular
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

MGI:1355274

InterPro

IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase

PFAM

PF01088

PRINTS

PR00707

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9JKB1

PhosphoSite

PhosphoSite-Q9JKB1

TrEMBL

UniProt Splice Variant

Entrez Gene

50933

UniGene

RefSeq

NP_057932

MGI ID

MGI Symbol

Uchl3

OMIM

CCDS

CCDS27313

HPRD

IMGT

EMBL

AB033370 AF247358 BC048481

GenPept

AAF64193 AAH48481 BAB20094