InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Macf1

Summary

InnateDB Protein

IDBP-185425.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Macf1

Protein Name

microtubule-actin crosslinking factor 1

Synonyms

ABP620; Acf7; Aclp7; MACF; mKIAA0465; R74989;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000080755

InnateDB Gene

IDBG-185419 (Macf1)

Protein Structure

UniProt Annotation

Function

Isoform 2 is a F-actin-binding protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. Plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane. Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics. May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4. Plays a key role in wound healing and epidermal cell migration. Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate MT dynamics and polarize hair follicle stem cells. {ECO:0000269PubMed:16815997, ECO:0000269PubMed:18854161, ECO:0000269PubMed:21295697}.

Subcellular Localization

Isoform 2: Cytoplasm. Cytoplasm, cytoskeleton. Golgi apparatus {ECO:0000250}. Cell membrane {ECO:0000250}. Cell projection, ruffle membrane {ECO:0000250}. Note=APC controls its localization to the cell membrane which is critical for its function in microtubule stabilization (By similarity). Localizes to the tips of microtubules. The phosphorylated form is found in the cytoplasm while the non- phosphorylated form associates with the microtubules. {ECO:0000250}.Isoform 1: Cytoplasm {ECO:0000250}. Golgi apparatus {ECO:0000250}.

Disease Associations

Tissue Specificity

Enriched in the hair follicle stem cells (at protein level). Isoform 1 and isoform 2 are ubiquitous expressed, with higher levels seen in lung, heart, thymus, spleen and brain. {ECO:0000269PubMed:16076900, ECO:0000269PubMed:16815997, ECO:0000269PubMed:21295697}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.
They are also associated with 34 interaction(s) predicted by orthology.

Experimentally validated
Total	2 [view]
Protein-Protein	2 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	34 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003824	catalytic activity
GO:0005509	calcium ion binding
GO:0005515	protein binding

Biological Process

GO:0007050	cell cycle arrest
GO:0044237	cellular metabolic process

Cellular Component

Protein Structure and Domains

PDB ID

MGI:108559

InterPro

IPR001715 Calponin homology domain
IPR002017 Spectrin repeat
IPR002048 EF-hand domain
IPR003108 Growth-arrest-specific protein 2 domain
IPR010997 HRDC-like
IPR018159 Spectrin/alpha-actinin
IPR022613 Calmodulin-regulated spectrin-associated protein, CH domain
IPR024930 Skp domain

PFAM