InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Nanog

Summary

InnateDB Protein

IDBP-185737.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Nanog

Protein Name

Nanog homeobox

Synonyms

2410002E02Rik; ecat4; ENK;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000012540

InnateDB Gene

IDBG-185735 (Nanog)

Protein Structure

UniProt Annotation

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. {ECO:0000269PubMed:12787504, ECO:0000269PubMed:12787505, ECO:0000269PubMed:14728807, ECO:0000269PubMed:15502159, ECO:0000269PubMed:16518401, ECO:0000269PubMed:16791199, ECO:0000269PubMed:16801560, ECO:0000269PubMed:21915945, ECO:0000269PubMed:22988117}.

Subcellular Localization

Nucleus {ECO:0000255PROSITE- ProRule:PRU00108, ECO:0000269PubMed:15582778}.

Disease Associations

Tissue Specificity

Not expressed in oocytes and spermatogonia (at protein level). Not expressed in many somatic organs, ovary, testis, fibroblast and hematopoietic cell lines. {ECO:0000269PubMed:12787504, ECO:0000269PubMed:12787505, ECO:0000269PubMed:15108323, ECO:0000269PubMed:15939376}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 288 experimentally validated interaction(s) in this database.
They are also associated with 5 interaction(s) predicted by orthology.

Experimentally validated
Total	288 [view]
Protein-Protein	278 [view]
Protein-DNA	10 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	5 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001158	enhancer sequence-specific DNA binding
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0003714	transcription corepressor activity
GO:0005515	protein binding
GO:0043565	sequence-specific DNA binding
GO:0044212	transcription regulatory region DNA binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0001710	mesodermal cell fate commitment
GO:0001714	endodermal cell fate specification
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0008283	cell proliferation
GO:0008284	positive regulation of cell proliferation
GO:0008406	gonad development
GO:0009790	embryo development
GO:0009880	embryonic pattern specification
GO:0010033	response to organic substance
GO:0010454	negative regulation of cell fate commitment
GO:0010468	regulation of gene expression
GO:0017145	stem cell division
GO:0019827	stem cell maintenance
GO:0030154	cell differentiation
GO:0030514	negative regulation of BMP signaling pathway
GO:0032526	response to retinoic acid
GO:0035019	somatic stem cell maintenance
GO:0042664	negative regulation of endodermal cell fate specification
GO:0045595	regulation of cell differentiation
GO:0045596	negative regulation of cell differentiation
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045931	positive regulation of mitotic cell cycle
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0048863	stem cell differentiation