InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Cul7

Summary

InnateDB Protein

IDBP-187623.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Cul7

Protein Name

cullin 7

Synonyms

2510004L20Rik; AA409809; C230011P08Rik; p185; p193;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000049128

InnateDB Gene

IDBG-187621 (Cul7)

Protein Structure

UniProt Annotation

Function

Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5. Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1. Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain. Mediates ubiquitination and degradation of IRS1 in a mTOR- dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradatation, thereby affecting cell proliferation and differentiation. Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development. Does not promote polyubiquitination and proteasomal degradation of p53/TP53. While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions (By similarity). Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation. {ECO:0000250, ECO:0000269PubMed:12904573}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}. Golgi apparatus {ECO:0000250}. Note=Colocalizes with FBXW8 at the Golgi apparatus in neurons; localization to Golgi is mediated by OBSL1. During mitosis, localizes to the mitotic apparatus. CCDC8 is required for centrosomal location (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 7 experimentally validated interaction(s) in this database.
They are also associated with 23 interaction(s) predicted by orthology.

Experimentally validated
Total	7 [view]
Protein-Protein	7 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	23 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005488	binding
GO:0005515	protein binding
GO:0031625	ubiquitin protein ligase binding

Biological Process

GO:0000226	microtubule cytoskeleton organization
GO:0000281	mitotic cytokinesis
GO:0001570	vasculogenesis
GO:0001837	epithelial to mesenchymal transition
GO:0001890	placenta development
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0006915	apoptotic process
GO:0007030	Golgi organization
GO:0007088	regulation of mitosis
GO:0016567	protein ubiquitination
GO:0042981	regulation of apoptotic process
GO:0045601	regulation of endothelial cell differentiation
GO:0050775	positive regulation of dendrite morphogenesis

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005813	centrosome
GO:0031461	cullin-RING ubiquitin ligase complex
GO:0031467	Cul7-RING ubiquitin ligase complex
GO:0048471	perinuclear region of cytoplasm
GO:1990393	3M complex

Protein Structure and Domains

PDB ID

MGI:1913765

InterPro

IPR001373 Cullin, N-terminal
IPR004939 Anaphase-promoting complex, subunit 10/DOC domain
IPR008979 Galactose-binding domain-like
IPR016024 Armadillo-type fold
IPR016158 Cullin homology
IPR019559 Cullin protein, neddylation domain
IPR021097 CPH domain

PFAM

PF00888
PF03256
PF10557
PF11515

PRINTS

PIRSF

SMART

SM00182
SM00884

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q8VE73