InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: E2f8

Summary

InnateDB Protein

IDBP-187714.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

E2f8

Protein Name

E2F transcription factor 8

Synonyms

4432406C08Rik; AA410048;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000056778

InnateDB Gene

IDBG-187710 (E2f8)

Protein Structure

UniProt Annotation

Function

Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. {ECO:0000269PubMed:18194653, ECO:0000269PubMed:22516201, ECO:0000269PubMed:22903062, ECO:0000269PubMed:23064264, ECO:0000269PubMed:23064266}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:15722552}.

Disease Associations

Tissue Specificity

Highly expressed in liver, skin, thymus and testis. Expressed in trophoblast giant cells throughout placenta development (at protein level). {ECO:0000269PubMed:15722552, ECO:0000269PubMed:22516201, ECO:0000269PubMed:23064266}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 3 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	3 [view]
Protein-Protein	3 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000978	RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001047	core promoter binding
GO:0001078	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription
GO:0003677	DNA binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0003714	transcription corepressor activity
GO:0005515	protein binding
GO:0042802	identical protein binding
GO:0042803	protein homodimerization activity

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0001890	placenta development
GO:0002040	sprouting angiogenesis
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0008283	cell proliferation
GO:0032466	negative regulation of cytokinesis
GO:0032877	positive regulation of DNA endoreduplication
GO:0033301	cell cycle comprising mitosis without cytokinesis
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0060707	trophoblast giant cell differentiation
GO:0060718	chorionic trophoblast cell differentiation
GO:0070365	hepatocyte differentiation