InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Lin28a

Summary

InnateDB Protein

IDBP-195577.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Lin28a

Protein Name

lin-28 homolog A (C. elegans)

Synonyms

AL024421; ENSMUSG00000070700; Gm10299; Lin-28; lin-28A; Lin28; Tex17;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000050488

InnateDB Gene

IDBG-195575 (Lin28a)

Protein Structure

UniProt Annotation

Function

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, Lin28a down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'- GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. {ECO:0000269PubMed:17473174, ECO:0000269PubMed:18292307, ECO:0000269PubMed:18566191, ECO:0000269PubMed:18604195}.

Subcellular Localization

Cytoplasm. Nucleus, nucleolus. Note=Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules (By similarity). Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes. {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in embryonic stem cells (ES cells), spermatagonia and testis. Expressed in numerous epithelial tissues including the epithelia of the small intestine, the intralobular duct epithelium of the mammary gland and the epithelia of Henle's loop in the kidney and in the collecting duct (at protein level). Also expressed in the myocardium and skeletal muscle (at protein level). {ECO:0000269PubMed:11279525, ECO:0000269PubMed:12798299, ECO:0000269PubMed:14643679, ECO:0000269PubMed:15722555}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 18 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	18 [view]
Protein-Protein	17 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0003729	mRNA binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0031369	translation initiation factor binding
GO:0035198	miRNA binding

Biological Process

GO:0006355	regulation of transcription, DNA-templated
GO:0007281	germ cell development
GO:0010586	miRNA metabolic process
GO:0010587	miRNA catabolic process
GO:0019827	stem cell maintenance
GO:0031054	pre-miRNA processing
GO:0031123	RNA 3'-end processing
GO:0045666	positive regulation of neuron differentiation
GO:0045686	negative regulation of glial cell differentiation
GO:0045727	positive regulation of translation
GO:0048863	stem cell differentiation
GO:0060964	regulation of gene silencing by miRNA