InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rhoa

Summary

InnateDB Protein

IDBP-199635.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rhoa

Protein Name

ras homolog gene family, member A

Synonyms

Arha; Arha1; Arha2;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000007959

InnateDB Gene

IDBG-199633 (Rhoa)

Protein Structure

UniProt Annotation

Function

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Required for the apical junction formation of keratinocyte cell-cell adhesion. {ECO:0000250, ECO:0000269PubMed:11777936, ECO:0000269PubMed:20974804}.

Subcellular Localization

Cell membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cleavage furrow {ECO:0000250}. Cytoplasm, cell cortex {ECO:0000250}. Midbody {ECO:0000250}. Cell projection, lamellipodium {ECO:0000269PubMed:11777936, ECO:0000269PubMed:21543326}. Note=Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner (By similarity). Localized to cell-cell contacts in calcium-treated keratinocytes. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 43 experimentally validated interaction(s) in this database.
They are also associated with 100 interaction(s) predicted by orthology.

Experimentally validated
Total	43 [view]
Protein-Protein	41 [view]
Protein-DNA	0
Protein-RNA	1 [view]
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	100 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0019003	GDP binding
GO:0019904	protein domain specific binding

Biological Process

GO:0000902	cell morphogenesis
GO:0001666	response to hypoxia
GO:0006184	GTP catabolic process
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0007010	cytoskeleton organization
GO:0007155	cell adhesion
GO:0007160	cell-matrix adhesion
GO:0007165	signal transduction
GO:0007229	integrin-mediated signaling pathway
GO:0007264	small GTPase mediated signal transduction
GO:0007266	Rho protein signal transduction
GO:0007519	skeletal muscle tissue development
GO:0008064	regulation of actin polymerization or depolymerization
GO:0009612	response to mechanical stimulus
GO:0009749	response to glucose
GO:0015031	protein transport
GO:0021795	cerebral cortex cell migration
GO:0021861	forebrain radial glial cell differentiation
GO:0030036	actin cytoskeleton organization
GO:0030154	cell differentiation
GO:0030307	positive regulation of cell growth
GO:0030334	regulation of cell migration
GO:0030335	positive regulation of cell migration
GO:0030521	androgen receptor signaling pathway
GO:0030838	positive regulation of actin filament polymerization
GO:0031098	stress-activated protein kinase signaling cascade
GO:0032467	positive regulation of cytokinesis
GO:0033144	negative regulation of intracellular steroid hormone receptor signaling pathway
GO:0033688	regulation of osteoblast proliferation
GO:0036089	cleavage furrow formation
GO:0042493	response to drug
GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043124	negative regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043149	stress fiber assembly
GO:0043200	response to amino acid
GO:0043280	positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043297	apical junction assembly
GO:0043524	negative regulation of neuron apoptotic process
GO:0043525	positive regulation of neuron apoptotic process
GO:0043931	ossification involved in bone maturation
GO:0045471	response to ethanol
GO:0045665	negative regulation of neuron differentiation
GO:0045666	positive regulation of neuron differentiation
GO:0045727	positive regulation of translation
GO:0045785	positive regulation of cell adhesion
GO:0045907	positive regulation of vasoconstriction
GO:0045987	positive regulation of smooth muscle contraction
GO:0048812	neuron projection morphogenesis
GO:0050773	regulation of dendrite development
GO:0051384	response to glucocorticoid
GO:0051496	positive regulation of stress fiber assembly
GO:0051924	regulation of calcium ion transport
GO:0060548	negative regulation of cell death
GO:0061383	trabecula morphogenesis
GO:0071803	positive regulation of podosome assembly
GO:2000177	regulation of neural precursor cell proliferation

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005829	cytosol
GO:0005856	cytoskeleton
GO:0005886	plasma membrane
GO:0005938	cell cortex
GO:0016020	membrane
GO:0030027	lamellipodium
GO:0030424	axon
GO:0030496	midbody
GO:0032154	cleavage furrow
GO:0032587	ruffle membrane

Protein Structure and Domains

PDB ID

MGI:1096342

InterPro

IPR000795 Elongation factor, GTP-binding domain
IPR001806 Small GTPase superfamily
IPR003578 Small GTPase superfamily, Rho type
IPR003579 Small GTPase superfamily, Rab type
IPR005225 Small GTP-binding protein domain
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR013684 Mitochondrial Rho-like
IPR020849 Small GTPase superfamily, Ras type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00009
PF00071
PF00025
PF08477

PRINTS

PR00315
PR00449
PR00328

PIRSF

SMART

SM00174
SM00175
SM00173

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt