InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PKM2

Summary

InnateDB Protein

IDBP-20131.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PKM2

Protein Name

pyruvate kinase, muscle

Synonyms

CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1;

Species

Homo sapiens

Ensembl Protein

ENSP00000373745

InnateDB Gene

IDBG-20117 (PKM2)

Protein Structure

UniProt Annotation

Function

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. {ECO:0000269PubMed:17308100, ECO:0000269PubMed:18191611, ECO:0000269PubMed:21620138}.

Subcellular Localization

Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.

Disease Associations

Tissue Specificity

Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. {ECO:0000269PubMed:18191611}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 137 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.

Experimentally validated
Total	137 [view]
Protein-Protein	134 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	9 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0004743	pyruvate kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0023026	MHC class II protein complex binding
GO:0030955	potassium ion binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0005975	carbohydrate metabolic process
GO:0006006	glucose metabolic process
GO:0006096	glycolytic process
GO:0012501	programmed cell death
GO:0016310	phosphorylation
GO:0044281	small molecule metabolic process

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR001697 Pyruvate kinase
IPR011037 Pyruvate kinase-like, insert domain
IPR015793 Pyruvate kinase, barrel
IPR015795 Pyruvate kinase, C-terminal
IPR015813 Pyruvate/Phosphoenolpyruvate kinase-like domain

PFAM

PF00224
PF02887

PRINTS

PR01050

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P14618