InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Supt6h

Summary

InnateDB Protein

IDBP-202418.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Supt6h

Protein Name

suppressor of Ty 6 homolog (S. cerevisiae)

Synonyms

5131400N11Rik; AI132449; mKIAA0162; SPT6; Supt6h;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000002121

InnateDB Gene

IDBG-202416 (Supt6h)

Protein Structure

UniProt Annotation

Function

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A. {ECO:0000269PubMed:17234882, ECO:0000269PubMed:21518874, ECO:0000269PubMed:22843687, ECO:0000269PubMed:23503590}.

Subcellular Localization

Nucleus {ECO:0000305}.

Disease Associations

Tissue Specificity

Ubiquitously expressed.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 14 experimentally validated interaction(s) in this database.
They are also associated with 18 interaction(s) predicted by orthology.

Experimentally validated
Total	14 [view]
Protein-Protein	14 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	18 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0005515	protein binding
GO:0016788	hydrolase activity, acting on ester bonds
GO:0042393	histone binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0006139	nucleobase-containing compound metabolic process
GO:0006351	transcription, DNA-templated
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0006397	mRNA processing
GO:0008380	RNA splicing
GO:0010793	regulation of mRNA export from nucleus
GO:0032784	regulation of DNA-templated transcription, elongation
GO:0032968	positive regulation of transcription elongation from RNA polymerase II promoter
GO:0045191	regulation of isotype switching
GO:0050684	regulation of mRNA processing
GO:0051028	mRNA transport
GO:0051147	regulation of muscle cell differentiation
GO:0061086	negative regulation of histone H3-K27 methylation

Cellular Component

GO:0005634

nucleus

Protein Structure and Domains

PDB ID

MGI:107726

InterPro

IPR000980 SH2 domain
IPR003029 Ribosomal protein S1, RNA-binding domain
IPR006641 YqgF/RNase H-like domain
IPR012340 Nucleic acid-binding, OB-fold
IPR017072 Transcription elongation factor Spt6
IPR022967 RNA-binding domain, S1

PFAM

PF00017
PF14633
PF00575

PRINTS

PR00401

PIRSF

PIRSF036947

SMART

SM00252
SM00732
SM00316

TIGRFAMs

Post-translational Modifications

Modification

Cross-References