InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Ap2b1

Summary

InnateDB Protein

IDBP-205187.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Ap2b1

Protein Name

adaptor-related protein complex 2, beta 1 subunit

Synonyms

1300012O03Rik; AI788979;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000018875

InnateDB Gene

IDBG-205183 (Ap2b1)

Protein Structure

UniProt Annotation

Function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin- associated sorting proteins (CLASPs) are recognized by their [DE]- X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly (By similarity). {ECO:0000250}.

Subcellular Localization

Cell membrane {ECO:0000250}. Membrane, coated pit {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 23 experimentally validated interaction(s) in this database.
They are also associated with 45 interaction(s) predicted by orthology.

Experimentally validated
Total	23 [view]
Protein-Protein	23 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	45 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005488	binding
GO:0005515	protein binding
GO:0008565	protein transporter activity
GO:0030276	clathrin binding
GO:0032403	protein complex binding

Biological Process

GO:0006886	intracellular protein transport
GO:0006897	endocytosis
GO:0015031	protein transport
GO:0016192	vesicle-mediated transport
GO:0048268	clathrin coat assembly

Cellular Component

GO:0005802	trans-Golgi network
GO:0005886	plasma membrane
GO:0005905	coated pit
GO:0030117	membrane coat
GO:0030118	clathrin coat
GO:0030131	clathrin adaptor complex

Protein Structure and Domains

PDB ID

MGI:1919020

InterPro

IPR000225 Armadillo
IPR000357 HEAT
IPR002553 Clathrin/coatomer adaptor, adaptin-like, N-terminal
IPR008152 Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain
IPR009028 Coatomer/calthrin adaptor appendage, C-terminal subdomain
IPR013041 Coatomer/clathrin adaptor appendage, Ig-like subdomain
IPR015151 Beta-adaptin appendage, C-terminal subdomain
IPR016024 Armadillo-type fold
IPR016342 AP-1, 2,4 complex subunit beta

PFAM

PF00514
PF02985
PF01602
PF02883
PF09066

PRINTS

PIRSF

PIRSF002291

SMART

SM00185
SM00809
SM01020

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt