InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Nek2

Summary

InnateDB Protein

IDBP-208877.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Nek2

Protein Name

NIMA (never in mitosis gene a)-related expressed kinase 2

Synonyms

AA617254; C77054;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000027931

InnateDB Gene

IDBG-208875 (Nek2)

Protein Structure

UniProt Annotation

Function

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates and activates NEK11 in G1/S-arrested cells. {ECO:0000269PubMed:14668482, ECO:0000269PubMed:14697346}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:9187143}. Nucleus, nucleolus {ECO:0000250}. Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}. Chromosome, centromere {ECO:0000269PubMed:9187143}. Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Most abundantly expressed in testis. Low levels found in mid-gestation embryo, ovary, placenta, intestine, thymus and skin. Within the testis, expression restricted to germ cells with highest levels detected in spermatocytes at pachytene and diplotene stages. Also expressed in meiotic pachytene oocytes. {ECO:0000269PubMed:9187143, ECO:0000269PubMed:9434622}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.
They are also associated with 33 interaction(s) predicted by orthology.

Experimentally validated
Total	4 [view]
Protein-Protein	4 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	33 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0019903	protein phosphatase binding
GO:0046872	metal ion binding

Biological Process

GO:0000070	mitotic sister chromatid segregation
GO:0001824	blastocyst development
GO:0006468	protein phosphorylation
GO:0007059	chromosome segregation
GO:0007126	meiotic nuclear division
GO:0043392	negative regulation of DNA binding
GO:0046777	protein autophosphorylation
GO:0051299	centrosome separation
GO:0051988	regulation of attachment of spindle microtubules to kinetochore
GO:0090307	spindle assembly involved in mitosis
GO:1903126	negative regulation of centriole-centriole cohesion

Cellular Component

GO:0000776	kinetochore
GO:0000777	condensed chromosome kinetochore
GO:0000794	condensed nuclear chromosome
GO:0000922	spindle pole
GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0005813	centrosome
GO:0005874	microtubule
GO:0030496	midbody
GO:0043234	protein complex
GO:0045171	intercellular bridge

Protein Structure and Domains

PDB ID

MGI:109359

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt