InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Brca2

Summary

InnateDB Protein

IDBP-209589.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Brca2

Protein Name

breast cancer 2

Synonyms

Fancd1; RAB163;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000038576

InnateDB Gene

IDBG-209587 (Brca2)

Protein Structure

UniProt Annotation

Function

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Nucleus {ECO:0000250}.

Disease Associations

Tissue Specificity

Widely expressed. Highest expression in cerebellum, testis, ileum, appendix, epididymis, ovary and mammary gland. No expression in lung.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.
They are also associated with 64 interaction(s) predicted by orthology.

Experimentally validated
Total	8 [view]
Protein-Protein	8 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	64 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0002020	protease binding
GO:0003697	single-stranded DNA binding
GO:0005515	protein binding
GO:0010484	H3 histone acetyltransferase activity
GO:0010485	H4 histone acetyltransferase activity
GO:0043015	gamma-tubulin binding

Biological Process

GO:0000724	double-strand break repair via homologous recombination
GO:0000910	cytokinesis
GO:0001556	oocyte maturation
GO:0001833	inner cell mass cell proliferation
GO:0006281	DNA repair
GO:0006289	nucleotide-excision repair
GO:0006302	double-strand break repair
GO:0006310	DNA recombination
GO:0006355	regulation of transcription, DNA-templated
GO:0006974	cellular response to DNA damage stimulus
GO:0006978	DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
GO:0007141	male meiosis I
GO:0007283	spermatogenesis
GO:0007420	brain development
GO:0007569	cell aging
GO:0008283	cell proliferation
GO:0008585	female gonad development
GO:0008630	intrinsic apoptotic signaling pathway in response to DNA damage
GO:0010165	response to X-ray
GO:0010225	response to UV-C
GO:0010332	response to gamma radiation
GO:0030097	hemopoiesis
GO:0032465	regulation of cytokinesis
GO:0033600	negative regulation of mammary gland epithelial cell proliferation
GO:0042127	regulation of cell proliferation
GO:0042771	intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
GO:0043009	chordate embryonic development
GO:0043966	histone H3 acetylation
GO:0043967	histone H4 acetylation
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045931	positive regulation of mitotic cell cycle
GO:0048478	replication fork protection
GO:0051276	chromosome organization
GO:0051298	centrosome duplication

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005813	centrosome
GO:0030141	secretory granule
GO:0033593	BRCA2-MAGE-D1 complex
GO:0043234	protein complex

Protein Structure and Domains

PDB ID

MGI:109337

InterPro

IPR002093 BRCA2 repeat
IPR012340 Nucleic acid-binding, OB-fold
IPR015187 BRCA2, oligonucleotide/oligosaccharide-binding 1
IPR015188 BRCA2, oligonucleotide/oligosaccharide-binding 3
IPR015205 Tower
IPR015252 Breast cancer type 2 susceptibility protein, helical domain
IPR015525 Breast cancer type 2 susceptibility protein

PFAM

PF00634
PF09103
PF09104
PF09121
PF09169

PRINTS

PIRSF

PIRSF002397

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P97929