InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Brsk2

Summary

InnateDB Protein

IDBP-212426.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Brsk2

Protein Name

BR serine/threonine kinase 2

Synonyms

4833424K13Rik; SAD-A; SADA;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000074969

InnateDB Gene

IDBG-212422 (Brsk2)

Protein Structure

UniProt Annotation

Function

Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-175 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-261 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmatic reticulum (ER) stress. {ECO:0000269PubMed:15705853, ECO:0000269PubMed:17482548, ECO:0000269PubMed:20026642, ECO:0000269PubMed:22669945, ECO:0000269PubMed:22798068}.

Subcellular Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}. Endoplasmic reticulum {ECO:0000250}. Note=Detected at centrosomes during mitosis. Localizes to the endoplasmic reticulum in response to stress caused by tunicamycin (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Detected in pancreas islets and in brain (at protein level). Detected in brain and pancreas. {ECO:0000269PubMed:22798068}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 8 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	8 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000287	magnesium ion binding
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0019901	protein kinase binding
GO:0050321	tau-protein kinase activity

Biological Process

GO:0000086	G2/M transition of mitotic cell cycle
GO:0006468	protein phosphorylation
GO:0006887	exocytosis
GO:0007067	mitotic nuclear division
GO:0007409	axonogenesis
GO:0018105	peptidyl-serine phosphorylation
GO:0030010	establishment of cell polarity
GO:0030182	neuron differentiation
GO:0031532	actin cytoskeleton reorganization
GO:0048812	neuron projection morphogenesis
GO:0061178	regulation of insulin secretion involved in cellular response to glucose stimulus
GO:0070059	intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress

Cellular Component

GO:0005737	cytoplasm
GO:0005783	endoplasmic reticulum
GO:0005813	centrosome
GO:0048471	perinuclear region of cytoplasm

Protein Structure and Domains

PDB ID

MGI:1923020

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt