InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: GAPDH

Summary

InnateDB Protein

IDBP-236487.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

GAPDH

Protein Name

glyceraldehyde-3-phosphate dehydrogenase

Synonyms

G3PD; GAPD; HEL-S-162eP;

Species

Homo sapiens

Ensembl Protein

ENSP00000380070

InnateDB Gene

IDBG-14270 (GAPDH)

Protein Structure

UniProt Annotation

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. {ECO:0000250, ECO:0000269PubMed:11724794, ECO:0000269PubMed:23071094, ECO:0000269PubMed:3170585}.

Subcellular Localization

Cytoplasm, cytosol {ECO:0000269PubMed:12829261}. Nucleus {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000269PubMed:12829261}. Membrane {ECO:0000269PubMed:12829261}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 232 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	232 [view]
Protein-Protein	216 [view]
Protein-DNA	10 [view]
Protein-RNA	6 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004365	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
GO:0005515	protein binding
GO:0008017	microtubule binding
GO:0016620	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GO:0035605	peptidyl-cysteine S-nitrosylase activity
GO:0042802	identical protein binding
GO:0050661	NADP binding
GO:0051287	NAD binding

Biological Process

GO:0000226	microtubule cytoskeleton organization
GO:0005975	carbohydrate metabolic process
GO:0006006	glucose metabolic process
GO:0006094	gluconeogenesis
GO:0006096	glycolytic process
GO:0017148	negative regulation of translation
GO:0035606	peptidyl-cysteine S-trans-nitrosylation
GO:0044281	small molecule metabolic process
GO:0050821	protein stabilization
GO:0051402	neuron apoptotic process
GO:0055114	oxidation-reduction process
GO:0071346	cellular response to interferon-gamma

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005811	lipid particle
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0015630	microtubule cytoskeleton
GO:0016020	membrane
GO:0030529	ribonucleoprotein complex
GO:0048471	perinuclear region of cytoplasm
GO:0070062	extracellular vesicular exosome
GO:0097452	GAIT complex

Protein Structure and Domains

PDB ID

InterPro

IPR006424 Glyceraldehyde-3-phosphate dehydrogenase, type I
IPR020828 Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain
IPR020829 Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain
IPR020831 Glyceraldehyde/Erythrose phosphate dehydrogenase family

PFAM

PF00044
PF02800

PRINTS

PR00078

PIRSF

PIRSF000149

SMART

SM00846

TIGRFAMs

Post-translational Modifications

Modification

Cross-References