InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TRIM21

Summary

InnateDB Protein

IDBP-24925.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TRIM21

Protein Name

tripartite motif containing 21

Synonyms

RNF81; Ro/SSA; RO52; SSA; SSA1;

Species

Homo sapiens

Ensembl Protein

ENSP00000254436

InnateDB Gene

IDBG-24923 (TRIM21)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ('Thr- 187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. {ECO:0000269PubMed:16297862, ECO:0000269PubMed:16316627, ECO:0000269PubMed:16472766, ECO:0000269PubMed:16880511, ECO:0000269PubMed:18022694, ECO:0000269PubMed:18361920, ECO:0000269PubMed:18641315, ECO:0000269PubMed:18845142, ECO:0000269PubMed:19675099}.

Subcellular Localization

Cytoplasm. Nucleus. Cytoplasm, P-body. Note=Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5. Colocalizes with DCP2 in P-body.

Disease Associations

Tissue Specificity

Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 85 experimentally validated interaction(s) in this database.
They are also associated with 5 interaction(s) predicted by orthology.

Experimentally validated
Total	85 [view]
Protein-Protein	84 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	5 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0000209	protein polyubiquitination
GO:0006513	protein monoubiquitination
GO:0007049	cell cycle
GO:0016567	protein ubiquitination
GO:0031648	protein destabilization
GO:0032088	negative regulation of NF-kappaB transcription factor activity
GO:0032479	regulation of type I interferon production
GO:0032481	positive regulation of type I interferon production
GO:0032897	negative regulation of viral transcription
GO:0045087	innate immune response (InnateDB)
GO:0045787	positive regulation of cell cycle
GO:0046598	positive regulation of viral entry into host cell
GO:0051091	positive regulation of sequence-specific DNA binding transcription factor activity
GO:0051865	protein autoubiquitination
GO:0070206	protein trimerization
GO:0090086	negative regulation of protein deubiquitination
GO:1902187	negative regulation of viral release from host cell

Cellular Component

GO:0000932	cytoplasmic mRNA processing body
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0019005	SCF ubiquitin ligase complex
GO:0030529	ribonucleoprotein complex

Protein Structure and Domains

PDB ID

InterPro

IPR000315 Zinc finger, B-box
IPR001841 Zinc finger, RING-type
IPR001870 B30.2/SPRY domain
IPR003613 U box domain
IPR003877 SPRY domain
IPR003879 Butyrophylin-like
IPR006574 SPRY-associated
IPR008985 Concanavalin A-like lectin/glucanases superfamily
IPR018957 Zinc finger, C3HC4 RING-type
IPR020457 Zinc finger, B-box, chordata

PFAM