InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Chst4

Summary

InnateDB Protein

IDBP-262851.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Chst4

Protein Name

carbohydrate (chondroitin 6/keratan) sulfotransferase 4

Synonyms

Gn6st-2; GST-3; HEC-GlcNAc6ST;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000104845

InnateDB Gene

IDBG-191695 (Chst4)

Protein Structure

UniProt Annotation

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of SELL ligand sialyl 6-sulfo Lewis X on SELL counter-receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location. {ECO:0000269PubMed:10435581, ECO:0000269PubMed:11520459, ECO:0000269PubMed:14593101, ECO:0000269PubMed:14597732, ECO:0000269PubMed:15111310}.

Subcellular Localization

Golgi apparatus membrane {ECO:0000269PubMed:15111310}; Single-pass type II membrane protein {ECO:0000269PubMed:15111310}.

Disease Associations

Tissue Specificity

Specifically expressed in HEV. {ECO:0000269PubMed:10435581}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001517	N-acetylglucosamine 6-O-sulfotransferase activity
GO:0008146	sulfotransferase activity

Biological Process

GO:0005975	carbohydrate metabolic process
GO:0006044	N-acetylglucosamine metabolic process
GO:0006477	protein sulfation
GO:0006790	sulfur compound metabolic process
GO:0006954	inflammatory response
GO:0050901	leukocyte tethering or rolling