InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CPSF3

Summary

InnateDB Protein

IDBP-27631.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CPSF3

Protein Name

cleavage and polyadenylation specific factor 3, 73kDa

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000238112

InnateDB Gene

IDBG-27627 (CPSF3)

Protein Structure

UniProt Annotation

Function

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. {ECO:0000269PubMed:14749727, ECO:0000269PubMed:15037765, ECO:0000269PubMed:17128255, ECO:0000269PubMed:18688255}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:15037765}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 43 experimentally validated interaction(s) in this database.

Experimentally validated
Total	43 [view]
Protein-Protein	43 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003723	RNA binding
GO:0004521	endoribonuclease activity
GO:0005515	protein binding
GO:0008409	5'-3' exonuclease activity
GO:0046872	metal ion binding

Biological Process

GO:0000398	mRNA splicing, via spliceosome
GO:0006366	transcription from RNA polymerase II promoter
GO:0006369	termination of RNA polymerase II transcription
GO:0006378	mRNA polyadenylation
GO:0006379	mRNA cleavage
GO:0006398	histone mRNA 3'-end processing
GO:0006406	mRNA export from nucleus
GO:0008380	RNA splicing
GO:0010467	gene expression
GO:0031124	mRNA 3'-end processing
GO:0090305	nucleic acid phosphodiester bond hydrolysis
GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic

Cellular Component

GO:0005654	nucleoplasm
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex
GO:0030529	ribonucleoprotein complex

Protein Structure and Domains

PDB ID

InterPro

IPR001279 Beta-lactamase-like
IPR011108 RNA-metabolising metallo-beta-lactamase
IPR021718 Pre-mRNA 3\'-end-processing endonuclease polyadenylation factor C-term
IPR022712 Beta-Casp domain

PFAM

PF00753
PF07521
PF11718
PF10996

PRINTS

PIRSF

SMART

SM00849
SM01098
SM01027

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9UKF6