InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: THOC1

Summary

InnateDB Protein

IDBP-284.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

THOC1

Protein Name

THO complex 1

Synonyms

HPR1; P84; P84N5;

Species

Homo sapiens

Ensembl Protein

ENSP00000261600

InnateDB Gene

IDBG-282 (THOC1)

Protein Structure

UniProt Annotation

Function

Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.

Subcellular Localization

Isoform 1: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.Isoform 2: Cytoplasm.

Disease Associations

Tissue Specificity

Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. {ECO:0000269PubMed:15358532, ECO:0000269PubMed:15833825}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 64 experimentally validated interaction(s) in this database.

Experimentally validated
Total	64 [view]
Protein-Protein	60 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0005515	protein binding

Biological Process

GO:0000018	regulation of DNA recombination
GO:0006351	transcription, DNA-templated
GO:0006396	RNA processing
GO:0006397	mRNA processing
GO:0006406	mRNA export from nucleus
GO:0006915	apoptotic process
GO:0007165	signal transduction
GO:0008380	RNA splicing
GO:0031297	replication fork processing
GO:0032784	regulation of DNA-templated transcription, elongation
GO:0032786	positive regulation of DNA-templated transcription, elongation
GO:0046784	intronless viral mRNA export from host nucleus
GO:0048297	negative regulation of isotype switching to IgA isotypes
GO:2000002	negative regulation of DNA damage checkpoint

Cellular Component

GO:0000346	transcription export complex
GO:0000347	THO complex
GO:0000445	THO complex part of transcription export complex
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0016363	nuclear matrix
GO:0016607	nuclear speck
GO:0045171	intercellular bridge

Protein Structure and Domains

PDB ID

InterPro

IPR000488 Death domain
IPR011029 Death-like domain
IPR021861 THO complex, subunit THOC1
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase-like

PFAM

PF00531
PF11957

PRINTS

PIRSF

SMART

SM00005

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q96FV9