InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ROCK2

Summary

InnateDB Protein

IDBP-29659.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ROCK2

Protein Name

Rho-associated, coiled-coil containing protein kinase 2

Synonyms

ROCK-II;

Species

Homo sapiens

Ensembl Protein

ENSP00000317985

InnateDB Gene

IDBG-29657 (ROCK2)

Protein Structure

UniProt Annotation

Function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation. {ECO:0000269PubMed:10579722, ECO:0000269PubMed:15699075, ECO:0000269PubMed:16574662, ECO:0000269PubMed:17015463, ECO:0000269PubMed:19131646, ECO:0000269PubMed:19997641, ECO:0000269PubMed:21084279, ECO:0000269PubMed:21147781}.

Subcellular Localization

Cytoplasm. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic, and associated with actin microfilaments and the plasma membrane. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 22 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	22 [view]
Protein-Protein	21 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005198	structural molecule activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0017048	Rho GTPase binding
GO:0044822	poly(A) RNA binding
GO:0046872	metal ion binding

Biological Process

GO:0000910	cytokinesis
GO:0001843	neural tube closure
GO:0006468	protein phosphorylation
GO:0006939	smooth muscle contraction
GO:0007411	axon guidance
GO:0008625	extrinsic apoptotic signaling pathway via death domain receptors
GO:0010825	positive regulation of centrosome duplication
GO:0016525	negative regulation of angiogenesis
GO:0030036	actin cytoskeleton organization
GO:0030155	regulation of cell adhesion
GO:0032956	regulation of actin cytoskeleton organization
GO:0035556	intracellular signal transduction
GO:0042752	regulation of circadian rhythm
GO:0045616	regulation of keratinocyte differentiation
GO:0048511	rhythmic process
GO:0051298	centrosome duplication
GO:0051492	regulation of stress fiber assembly
GO:0051893	regulation of focal adhesion assembly
GO:2000114	regulation of establishment of cell polarity
GO:2000145	regulation of cell motility

Cellular Component

GO:0005634	nucleus
GO:0005813	centrosome
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0031616	spindle pole centrosome
GO:0036464	cytoplasmic ribonucleoprotein granule

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR000961 AGC-kinase, C-terminal
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR001849 Pleckstrin homology domain
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR010978 tRNA-binding arm
IPR011009 Protein kinase-like domain
IPR015008 Rho binding domain
IPR017892 Protein kinase, C-terminal
IPR020635 Tyrosine-protein kinase, catalytic domain
IPR020684 Rho-associated protein kinase 1/2

PFAM

PF00069
PF07714
PF00169
PF00130
PF08912
PF00433

PRINTS

PR00109

PIRSF

PIRSF037568

SMART

SM00133
SM00233
SM00109
SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References