Version 5.4
Homo sapiens Protein: P4HTM
Summary
InnateDB Protein IDBP-33891.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol P4HTM
Protein Name prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Synonyms
Species Homo sapiens
Ensembl Protein ENSP00000341422
InnateDB Gene IDBG-33889 (P4HTM)
Protein Structure
UniProt Annotation
Function Catalyzes the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. {ECO:0000269PubMed:17726031}.
Subcellular Localization Endoplasmic reticulum membrane {ECO:0000269PubMed:12163023, ECO:0000269PubMed:17726031}; Single-pass type II membrane protein {ECO:0000269PubMed:12163023, ECO:0000269PubMed:17726031}.
Disease Associations
Tissue Specificity Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts. {ECO:0000269PubMed:12163023, ECO:0000269PubMed:17726031}.
Comments
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 6 experimentally validated interaction(s) in this database.
Experimentally validated
Total 6 [view]
Protein-Protein 6 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Gene Ontology

Molecular Function
Accession GO Term
GO:0005506 iron ion binding
GO:0005509 calcium ion binding
GO:0016491 oxidoreductase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0031418 L-ascorbic acid binding
Biological Process
GO:0055114 oxidation-reduction process
Cellular Component
GO:0005789 endoplasmic reticulum membrane
GO:0016021 integral component of membrane
Protein Structure and Domains
PDB ID
InterPro IPR005123 Oxoglutarate/iron-dependent dioxygenase
IPR006620 Prolyl 4-hydroxylase, alpha subunit
PFAM PF03171
PF13640
PRINTS
PIRSF
SMART SM00702
TIGRFAMs
Post-translational Modifications
Modification
Cross-References
SwissProt Q9NXG6
PhosphoSite PhosphoSite-Q9NXG6
TrEMBL V9GYK5
UniProt Splice Variant
Entrez Gene 54681
UniGene Hs.654944
RefSeq NP_808807
HUGO HGNC:28858
OMIM 614584
CCDS CCDS2781
HPRD 17842
IMGT
EMBL AC137630 AK000269 AL713728 AY198406 BC000580 BC011710 BC047566 BC060321
GenPept AAH00580 AAH11710 AAH47566 AAH60321 AAO43431 BAA91045 CAD28518

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca