InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RAPGEF3

Summary

InnateDB Protein

IDBP-373339.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RAPGEF3

Protein Name

Rap guanine nucleotide exchange factor (GEF) 3

Synonyms

bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275;

Species

Homo sapiens

Ensembl Protein

ENSP00000395708

InnateDB Gene

IDBG-28934 (RAPGEF3)

Protein Structure

UniProt Annotation

Function

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho- mediated signaling. Required for the actin rearrangement at cell- cell junctions, such as stress fibers and junctional actin. {ECO:0000269PubMed:10777494, ECO:0000269PubMed:21840392, ECO:0000269PubMed:9853756}.

Subcellular Localization

Endomembrane system {ECO:0000269PubMed:10777494}.

Disease Associations

Tissue Specificity

Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. {ECO:0000269PubMed:9856955}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 12 experimentally validated interaction(s) in this database.

Experimentally validated
Total	12 [view]
Protein-Protein	12 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005085	guanyl-nucleotide exchange factor activity
GO:0005515	protein binding
GO:0008603	cAMP-dependent protein kinase regulator activity
GO:0017034	Rap guanyl-nucleotide exchange factor activity
GO:0030552	cAMP binding

Biological Process

GO:0001525	angiogenesis
GO:0006112	energy reserve metabolic process
GO:0007165	signal transduction
GO:0007264	small GTPase mediated signal transduction
GO:0007596	blood coagulation
GO:0008283	cell proliferation
GO:0019933	cAMP-mediated signaling
GO:0030168	platelet activation
GO:0030822	positive regulation of cAMP catabolic process
GO:0032486	Rap protein signal transduction
GO:0032854	positive regulation of Rap GTPase activity
GO:0035556	intracellular signal transduction
GO:0044281	small molecule metabolic process
GO:0045766	positive regulation of angiogenesis
GO:0045859	regulation of protein kinase activity
GO:0050796	regulation of insulin secretion
GO:0051056	regulation of small GTPase mediated signal transduction
GO:0051496	positive regulation of stress fiber assembly
GO:0061028	establishment of endothelial barrier
GO:0071320	cellular response to cAMP
GO:2000249	regulation of actin cytoskeleton reorganization

Cellular Component

GO:0005622	intracellular
GO:0005886	plasma membrane
GO:0005952	cAMP-dependent protein kinase complex
GO:0012505	endomembrane system
GO:0016020	membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR000591 DEP domain
IPR000595 Cyclic nucleotide-binding domain
IPR000651 Ras-like guanine nucleotide exchange factor, N-terminal
IPR001895 Ras guanine-nucleotide exchange factors catalytic domain
IPR002373 cAMP/cGMP-dependent protein kinase
IPR018490 Cyclic nucleotide-binding-like
IPR023578 Ras guanine nucleotide exchange factor domain
IPR029071 Ubiquitin-related domain

PFAM

PF00610
PF00027
PF00618
PF00617

PRINTS

PR00103

PIRSF

SMART

SM00049
SM00100
SM00229
SM00147

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt