InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CDK12

Summary

InnateDB Protein

IDBP-377066.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CDK12

Protein Name

cyclin-dependent kinase 12

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000407720

InnateDB Gene

IDBG-45681 (CDK12)

Protein Structure

UniProt Annotation

Function

Cyclin-dependent kinase that phosphorylates the C- terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors. {ECO:0000269PubMed:11683387, ECO:0000269PubMed:19651820, ECO:0000269PubMed:20952539, ECO:0000269PubMed:22012619, ECO:0000269PubMed:24662513}.

Subcellular Localization

Nucleus. Nucleus speckle. Note=Colocalized with nuclear speckles throughout interphase.

Disease Associations

Note=Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.

Tissue Specificity

Ubiquitously expressed. {ECO:0000269PubMed:11683387}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 26 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	26 [view]
Protein-Protein	24 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004693	cyclin-dependent protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008353	RNA polymerase II carboxy-terminal domain kinase activity
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0030332	cyclin binding

Biological Process

GO:0006397	mRNA processing
GO:0006468	protein phosphorylation
GO:0008380	RNA splicing
GO:0043405	regulation of MAP kinase activity
GO:0046777	protein autophosphorylation
GO:0051726	regulation of cell cycle
GO:0070816	phosphorylation of RNA polymerase II C-terminal domain

Cellular Component

GO:0002944	cyclin K-CDK12 complex
GO:0005634	nucleus
GO:0005730	nucleolus
GO:0016607	nuclear speck
GO:0019908	nuclear cyclin-dependent protein kinase holoenzyme complex

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt