InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TOP1MT

Summary

InnateDB Protein

IDBP-38319.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TOP1MT

Protein Name

topoisomerase (DNA) I, mitochondrial

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000328835

InnateDB Gene

IDBG-38317 (TOP1MT)

Protein Structure

UniProt Annotation

Function

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). {ECO:0000250}.

Subcellular Localization

Mitochondrion {ECO:0000269PubMed:11526219}.

Disease Associations

Tissue Specificity

Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver. {ECO:0000269PubMed:11526219}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.

Experimentally validated
Total	4 [view]
Protein-Protein	3 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003917	DNA topoisomerase type I activity
GO:0003918	DNA topoisomerase (ATP-hydrolyzing) activity

Biological Process

GO:0006200	ATP catabolic process
GO:0006260	DNA replication
GO:0006265	DNA topological change

Cellular Component

GO:0005634	nucleus
GO:0005694	chromosome
GO:0005739	mitochondrion
GO:0042645	mitochondrial nucleoid

Protein Structure and Domains

PDB ID

InterPro

IPR001631 DNA topoisomerase I
IPR008336 DNA topoisomerase I, DNA binding, eukaryotic-type
IPR011010 DNA breaking-rejoining enzyme, catalytic core
IPR013499 DNA topoisomerase I, eukaryotic-type
IPR013500 DNA topoisomerase I, catalytic core, eukaryotic-type

PFAM

PF02919
PF01028

PRINTS

PR00416

PIRSF

SMART

SM00435

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt