InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CHFR

Summary

InnateDB Protein

IDBP-385342.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CHFR

Protein Name

checkpoint with forkhead and ring finger domains

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000398735

InnateDB Gene

IDBG-66018 (CHFR)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. {ECO:0000269PubMed:10935642, ECO:0000269PubMed:11807090, ECO:0000269PubMed:11912157, ECO:0000269PubMed:14562038, ECO:0000269PubMed:14694445, ECO:0000269PubMed:18172500, ECO:0000269PubMed:19182791}.

Subcellular Localization

Nucleus, PML body {ECO:0000269PubMed:11912157, ECO:0000269PubMed:15467728, ECO:0000269PubMed:18172500}.

Disease Associations

Tissue Specificity

Ubiquitous. {ECO:0000269PubMed:10935642}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 49 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	49 [view]
Protein-Protein	41 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	8 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity

Biological Process

GO:0000209	protein polyubiquitination
GO:0007067	mitotic nuclear division
GO:0007093	mitotic cell cycle checkpoint
GO:0019941	modification-dependent protein catabolic process