InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SMARCA5

Summary

InnateDB Protein

IDBP-39366.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SMARCA5

Protein Name

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5

Synonyms

hISWI; hSNF2H; ISWI; SNF2H; WCRF135;

Species

Homo sapiens

Ensembl Protein

ENSP00000283131

InnateDB Gene

IDBG-39364 (SMARCA5)

Protein Structure

UniProt Annotation

Function

Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. {ECO:0000269PubMed:10880450, ECO:0000269PubMed:11980720, ECO:0000269PubMed:12198550, ECO:0000269PubMed:12434153, ECO:0000269PubMed:12972596, ECO:0000269PubMed:15543136, ECO:0000269PubMed:16603771}.

Subcellular Localization

Nucleus {ECO:0000255PROSITE- ProRule:PRU00624, ECO:0000269PubMed:12434153}.

Disease Associations

Tissue Specificity

Ubiquitously expressed.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 122 experimentally validated interaction(s) in this database.
They are also associated with 17 interaction(s) predicted by orthology.

Experimentally validated
Total	122 [view]
Protein-Protein	117 [view]
Protein-DNA	5 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	17 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0004386	helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016787	hydrolase activity
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
GO:0016887	ATPase activity
GO:0031491	nucleosome binding
GO:0042393	histone binding

Biological Process

GO:0000183	chromatin silencing at rDNA
GO:0006200	ATP catabolic process
GO:0006302	double-strand break repair
GO:0006333	chromatin assembly or disassembly
GO:0006334	nucleosome assembly
GO:0006338	chromatin remodeling
GO:0006352	DNA-templated transcription, initiation
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0016584	nucleosome positioning
GO:0034080	centromere-specific nucleosome assembly
GO:0043044	ATP-dependent chromatin remodeling

Cellular Component

GO:0000793	condensed chromosome
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005677	chromatin silencing complex
GO:0016589	NURF complex
GO:0031010	ISWI-type complex
GO:0031213	RSF complex
GO:0043596	nuclear replication fork

Protein Structure and Domains

PDB ID

InterPro

IPR000330 SNF2-related
IPR001005 SANT/Myb domain
IPR001650 Helicase, C-terminal
IPR006935 Helicase/UvrB domain
IPR009057 Homeodomain-like
IPR011545 DEAD/DEAH box helicase domain
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR015194 ISWI HAND domain
IPR015195 SLIDE domain
IPR021006 Histone deacetylase complex, subunit 2/3
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM