InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Klhl20

Summary

InnateDB Protein

IDBP-400349.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Klhl20

Protein Name

kelch-like 20 (Drosophila)

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000114044

InnateDB Gene

IDBG-201411 (Klhl20)

Protein Structure

UniProt Annotation

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination. Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm, perinuclear region {ECO:0000250}. Nucleus {ECO:0000250}. Golgi apparatus, trans-Golgi network {ECO:0000250}. Cell projection, axon {ECO:0000250}. Cell projection, dendrite {ECO:0000250}. Note=Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 18 interaction(s) predicted by orthology.

Predicted by orthology
Total	18 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003779	actin binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0019964	interferon-gamma binding

Biological Process

GO:0016567	protein ubiquitination
GO:0043066	negative regulation of apoptotic process
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process

Cellular Component

GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0016605	PML body
GO:0031463	Cul3-RING ubiquitin ligase complex
GO:0048471	perinuclear region of cytoplasm

Protein Structure and Domains

PDB ID

MGI:2444855

InterPro

IPR000210 BTB/POZ-like
IPR006652 Kelch repeat type 1
IPR011333 BTB/POZ fold
IPR011498 Kelch repeat type 2
IPR011705 BTB/Kelch-associated
IPR013069 BTB/POZ
IPR017096 Kelch-like protein, gigaxonin type

PFAM

PF01344
PF07646
PF07707
PF00651

PRINTS

PIRSF

PIRSF037037

SMART

SM00225
SM00612
SM00875

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt