InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PODXL

Summary

InnateDB Protein

IDBP-41800.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PODXL

Protein Name

podocalyxin-like

Synonyms

Gp200; PC; PCLP; PCLP-1;

Species

Homo sapiens

Ensembl Protein

ENSP00000367817

InnateDB Gene

IDBG-41798 (PODXL)

Protein Structure

UniProt Annotation

Function

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. {ECO:0000269PubMed:17616675, ECO:0000269PubMed:18456258}.

Subcellular Localization

Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus {ECO:0000250}. Membrane raft {ECO:0000250}. Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. Note=In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans- Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Glomerular epithelium cell (podocyte).

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 9 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	9 [view]
Protein-Protein	8 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding

Biological Process

GO:0007155	cell adhesion
GO:0007162	negative regulation of cell adhesion
GO:0016477	cell migration
GO:0022408	negative regulation of cell-cell adhesion
GO:0030335	positive regulation of cell migration
GO:0032534	regulation of microvillus assembly
GO:0033634	positive regulation of cell-cell adhesion mediated by integrin
GO:0050900	leukocyte migration
GO:0072015	glomerular visceral epithelial cell development
GO:0072175	epithelial tube formation

Cellular Component

GO:0001726	ruffle
GO:0005615	extracellular space
GO:0005737	cytoplasm
GO:0005886	plasma membrane
GO:0005887	integral component of plasma membrane
GO:0016324	apical plasma membrane
GO:0030027	lamellipodium
GO:0030175	filopodium
GO:0031528	microvillus membrane
GO:0036057	slit diaphragm
GO:0044297	cell body
GO:0045121	membrane raft
GO:0070062	extracellular vesicular exosome