InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RFWD3

Summary

InnateDB Protein

IDBP-42111.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RFWD3

Protein Name

ring finger and WD repeat domain 3

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000354361

InnateDB Gene

IDBG-42109 (RFWD3)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair. {ECO:0000269PubMed:20173098, ECO:0000269PubMed:21504906, ECO:0000269PubMed:21558276}.

Subcellular Localization

Nucleus. Nucleus, PML body. Cytoplasm. Note=In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies. In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair. This recruitment depends upon RPA2.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 26 experimentally validated interaction(s) in this database.

Experimentally validated
Total	26 [view]
Protein-Protein	26 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0002039	p53 binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding
GO:0097371	MDM2/MDM4 family protein binding

Biological Process

GO:0006281	DNA repair
GO:0010212	response to ionizing radiation
GO:0016567	protein ubiquitination
GO:0031571	mitotic G1 DNA damage checkpoint
GO:2000001	regulation of DNA damage checkpoint