InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: USP10

Summary

InnateDB Protein

IDBP-44811.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

USP10

Protein Name

ubiquitin specific peptidase 10

Synonyms

UBPO;

Species

Homo sapiens

Ensembl Protein

ENSP00000219473

InnateDB Gene

IDBG-44807 (USP10)

Protein Structure

UniProt Annotation

Function

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34- containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. {ECO:0000269PubMed:11439350, ECO:0000269PubMed:18632802, ECO:0000269PubMed:19398555, ECO:0000269PubMed:20096447, ECO:0000269PubMed:21962518}.

Subcellular Localization

Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.

Disease Associations

Tissue Specificity

Widely expressed. {ECO:0000269PubMed:11439350}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 46 experimentally validated interaction(s) in this database.

Experimentally validated
Total	46 [view]
Protein-Protein	46 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0002039	p53 binding
GO:0004197	cysteine-type endopeptidase activity
GO:0004221	ubiquitin thiolesterase activity
GO:0004843	ubiquitin-specific protease activity
GO:0005515	protein binding
GO:0036459	ubiquitinyl hydrolase activity
GO:0044325	ion channel binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0006281	DNA repair
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0006914	autophagy
GO:0010506	regulation of autophagy
GO:0016579	protein deubiquitination
GO:0030330	DNA damage response, signal transduction by p53 class mediator

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005769	early endosome
GO:0045111	intermediate filament cytoskeleton

Protein Structure and Domains

PDB ID

InterPro

IPR001394 Peptidase C19, ubiquitin carboxyl-terminal hydrolase
IPR009818 Ataxin-2, C-terminal
IPR028889 Ubiquitin carboxyl-terminal hydrolase-like domain

PFAM

PF00443
PF07145

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q14694

PhosphoSite