InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: USP13

Summary

InnateDB Protein

IDBP-480249.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

USP13

Protein Name

ubiquitin specific peptidase 13 (isopeptidase T-3)

Synonyms

IsoT-3; ISOT3;

Species

Homo sapiens

Ensembl Protein

ENSP00000417146

InnateDB Gene

IDBG-66443 (USP13)

Protein Structure

UniProt Annotation

Function

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. {ECO:0000269PubMed:17653289, ECO:0000269PubMed:21571647, ECO:0000269PubMed:21659512, ECO:0000269PubMed:21811243, ECO:0000269PubMed:21962518, ECO:0000269PubMed:22216260}.

Subcellular Localization

Disease Associations

Tissue Specificity

Highly expressed in ovary and testes.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 40 experimentally validated interaction(s) in this database.

Experimentally validated
Total	40 [view]
Protein-Protein	40 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004197	cysteine-type endopeptidase activity
GO:0004221	ubiquitin thiolesterase activity
GO:0004843	ubiquitin-specific protease activity
GO:0005515	protein binding
GO:0008242	omega peptidase activity
GO:0008270	zinc ion binding
GO:0031625	ubiquitin protein ligase binding
GO:0036459	ubiquitinyl hydrolase activity
GO:0043130	ubiquitin binding

Biological Process

GO:0006355	regulation of transcription, DNA-templated
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0006914	autophagy
GO:0008283	cell proliferation
GO:0010506	regulation of autophagy
GO:0030318	melanocyte differentiation
GO:0050821	protein stabilization
GO:0070536	protein K63-linked deubiquitination

Cellular Component

Protein Structure and Domains

PDB ID

InterPro

IPR000449 Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminal
IPR001394 Peptidase C19, ubiquitin carboxyl-terminal hydrolase
IPR001607 Zinc finger, UBP-type
IPR009060 UBA-like
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote
IPR016652 Ubiquitinyl hydrolase
IPR028889 Ubiquitin carboxyl-terminal hydrolase-like domain

PFAM

PF00627
PF00443
PF02148

PRINTS

PIRSF

PIRSF016308

SMART

SM00290
SM00165

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt