InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: UBE2K

Summary

InnateDB Protein

IDBP-484956.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

UBE2K

Protein Name

ubiquitin-conjugating enzyme E2K

Synonyms

E2-25K; HIP2; HYPG; LIG; UBC1;

Species

Homo sapiens

Ensembl Protein

ENSP00000421203

InnateDB Gene

IDBG-14649 (UBE2K)

Protein Structure

UniProt Annotation

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein- dependent degradation of RB1. {ECO:0000269PubMed:10634809, ECO:0000269PubMed:10675012, ECO:0000269PubMed:16714285, ECO:0000269PubMed:16868077, ECO:0000269PubMed:17873885, ECO:0000269PubMed:19906396, ECO:0000269PubMed:20061386, ECO:0000269PubMed:8702625}.

Subcellular Localization

Cytoplasm {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. {ECO:0000269PubMed:10634809, ECO:0000269PubMed:10675012, ECO:0000269PubMed:8702625}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 93 experimentally validated interaction(s) in this database.

Experimentally validated
Total	93 [view]
Protein-Protein	90 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016881	acid-amino acid ligase activity
GO:0031625	ubiquitin protein ligase binding
GO:0034450	ubiquitin-ubiquitin ligase activity

Biological Process

GO:0006511	ubiquitin-dependent protein catabolic process
GO:0070936	protein K48-linked ubiquitination

Cellular Component

GO:0005737	cytoplasm
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR000449 Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminal
IPR000608 Ubiquitin-conjugating enzyme, E2
IPR009060 UBA-like
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote
IPR016135 Ubiquitin-conjugating enzyme/RWD-like

PFAM

PF00627
PF00179

PRINTS

PIRSF

SMART

SM00165

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P61086