InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: FEN1

Summary

InnateDB Protein

IDBP-50441.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

FEN1

Protein Name

flap structure-specific endonuclease 1

Synonyms

FEN-1; MF1; RAD2;

Species

Homo sapiens

Ensembl Protein

ENSP00000305480

InnateDB Gene

IDBG-50439 (FEN1)

Protein Structure

UniProt Annotation

Function

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {ECO:0000255HAMAP-Rule:MF_03140, ECO:0000269PubMed:10744741, ECO:0000269PubMed:11986308, ECO:0000269PubMed:18443037, ECO:0000269PubMed:20729856, ECO:0000269PubMed:7961795, ECO:0000269PubMed:8621570}.

Subcellular Localization

Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.Isoform FENMIT: Mitochondrion {ECO:0000255HAMAP-Rule:MF_03140, ECO:0000269PubMed:23675412}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 58 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	58 [view]
Protein-Protein	54 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000287	magnesium ion binding
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0003690	double-stranded DNA binding
GO:0003824	catalytic activity
GO:0004518	nuclease activity
GO:0004519	endonuclease activity
GO:0004523	RNA-DNA hybrid ribonuclease activity
GO:0004527	exonuclease activity
GO:0005515	protein binding
GO:0008309	double-stranded DNA specific exodeoxyribonuclease activity
GO:0008409	5'-3' exonuclease activity
GO:0017108	5'-flap endonuclease activity
GO:0030145	manganese ion binding
GO:0048256	flap endonuclease activity

Biological Process

GO:0000278	mitotic cell cycle
GO:0000722	telomere maintenance via recombination
GO:0000723	telomere maintenance
GO:0000737	DNA catabolic process, endonucleolytic
GO:0000738	DNA catabolic process, exonucleolytic
GO:0006260	DNA replication
GO:0006271	DNA strand elongation involved in DNA replication
GO:0006281	DNA repair
GO:0006284	base-excision repair
GO:0006302	double-strand break repair
GO:0007613	memory
GO:0009650	UV protection
GO:0032201	telomere maintenance via semi-conservative replication
GO:0043137	DNA replication, removal of RNA primer
GO:0048015	phosphatidylinositol-mediated signaling
GO:0090305	nucleic acid phosphodiester bond hydrolysis
GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005730	nucleolus
GO:0005739	mitochondrion
GO:0016020	membrane

Protein Structure and Domains

PDB ID

InterPro

IPR006085 XPG N-terminal
IPR006086 XPG-I domain
IPR008918 Helix-hairpin-helix motif, class 2
IPR020045 5\'-3\' exonuclease, C-terminal domain
IPR029060 PIN domain-like

PFAM

PF00752
PF00867
PF01367
PF09293

PRINTS

PIRSF

SMART

SM00485
SM00484
SM00279

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P39748