InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Gene: FEN1

Summary

InnateDB Gene

IDBG-50439.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

FEN1

Gene Name

flap structure-specific endonuclease 1

Synonyms

FEN-1; MF1; RAD2

Species

Homo sapiens

Ensembl Gene

ENSG00000168496

Encoded Proteins

IDBP-50441

flap structure-specific endonuclease 1

IDBP-586487

flap structure-specific endonuclease 1

IDBP-718591

Protein Structure

Useful resources

Stemformatics EHFPI ImmGen

Entrez Gene

Summary

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]
The protein encoded by this gene removes 5\' overhanging flaps in DNA repair and processes the 5\' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5\' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Gene Information

Type

Protein coding

Genomic Location

Chromosome 11:61792637-61797244

Strand

Forward strand

Band

q12.2

Transcripts

ENST00000305885	ENSP00000305480
ENST00000535723	ENSP00000445692
ENST00000535307	ENSP00000460402

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 58 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	58 [view]
Protein-Protein	54 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000287	magnesium ion binding
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0003690	double-stranded DNA binding
GO:0003824	catalytic activity
GO:0004518	nuclease activity
GO:0004519	endonuclease activity
GO:0004523	RNA-DNA hybrid ribonuclease activity
GO:0004527	exonuclease activity
GO:0005515	protein binding
GO:0008309	double-stranded DNA specific exodeoxyribonuclease activity
GO:0008409	5'-3' exonuclease activity
GO:0017108	5'-flap endonuclease activity
GO:0030145	manganese ion binding
GO:0048256	flap endonuclease activity

Biological Process

GO:0000278	mitotic cell cycle
GO:0000722	telomere maintenance via recombination
GO:0000723	telomere maintenance
GO:0000737	DNA catabolic process, endonucleolytic
GO:0000738	DNA catabolic process, exonucleolytic
GO:0006260	DNA replication
GO:0006271	DNA strand elongation involved in DNA replication
GO:0006281	DNA repair
GO:0006284	base-excision repair
GO:0006302	double-strand break repair
GO:0007613	memory
GO:0009650	UV protection
GO:0032201	telomere maintenance via semi-conservative replication
GO:0043137	DNA replication, removal of RNA primer
GO:0048015	phosphatidylinositol-mediated signaling
GO:0090305	nucleic acid phosphodiester bond hydrolysis
GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic