InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PCNA

Summary

InnateDB Protein

IDBP-50803.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PCNA

Protein Name

proliferating cell nuclear antigen

Synonyms

ATLD2;

Species

Homo sapiens

Ensembl Protein

ENSP00000368458

InnateDB Gene

IDBG-50801 (PCNA)

Protein Structure

UniProt Annotation

Function

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. {ECO:0000269PubMed:18719106, ECO:0000269PubMed:19443450}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:24115439}. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 350 experimentally validated interaction(s) in this database.

Experimentally validated
Total	350 [view]
Protein-Protein	344 [view]
Protein-DNA	5 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000701	purine-specific mismatch base pair DNA N-glycosylase activity
GO:0003677	DNA binding
GO:0005515	protein binding
GO:0030337	DNA polymerase processivity factor activity
GO:0030971	receptor tyrosine kinase binding
GO:0032139	dinucleotide insertion or deletion binding
GO:0032405	MutLalpha complex binding
GO:0042802	identical protein binding
GO:0070182	DNA polymerase binding

Biological Process

GO:0000077	DNA damage checkpoint
GO:0000082	G1/S transition of mitotic cell cycle
GO:0000083	regulation of transcription involved in G1/S transition of mitotic cell cycle
GO:0000278	mitotic cell cycle
GO:0000722	telomere maintenance via recombination
GO:0000723	telomere maintenance
GO:0006260	DNA replication
GO:0006271	DNA strand elongation involved in DNA replication
GO:0006272	leading strand elongation
GO:0006275	regulation of DNA replication
GO:0006281	DNA repair
GO:0006283	transcription-coupled nucleotide-excision repair
GO:0006284	base-excision repair
GO:0006289	nucleotide-excision repair
GO:0006297	nucleotide-excision repair, DNA gap filling
GO:0006298	mismatch repair
GO:0007507	heart development
GO:0008283	cell proliferation
GO:0019985	translesion synthesis
GO:0030855	epithelial cell differentiation
GO:0032077	positive regulation of deoxyribonuclease activity
GO:0032201	telomere maintenance via semi-conservative replication
GO:0033993	response to lipid
GO:0046686	response to cadmium ion
GO:0048015	phosphatidylinositol-mediated signaling

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005663	DNA replication factor C complex
GO:0005737	cytoplasm
GO:0005813	centrosome
GO:0030896	checkpoint clamp complex
GO:0043596	nuclear replication fork
GO:0043626	PCNA complex
GO:0070062	extracellular vesicular exosome
GO:0070557	PCNA-p21 complex

Protein Structure and Domains

PDB ID

InterPro

IPR000730 Proliferating cell nuclear antigen, PCNA
IPR007268 Rad9/Ddc1
IPR022648 Proliferating cell nuclear antigen, PCNA, N-terminal
IPR022649 Proliferating cell nuclear antigen, PCNA, C-terminal

PFAM

PF04139
PF00705
PF02747

PRINTS

PR00339

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P12004