InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rc3h1

Summary

InnateDB Protein

IDBP-534960.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rc3h1

Protein Name

RING CCCH (C3H) domains 1

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000124871

InnateDB Gene

IDBG-201251 (Rc3h1)

Protein Structure

UniProt Annotation

Function

Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more mRNAs (PubMed:23663784). Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs. In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4/Ox40 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity. In resting or LPS- stimulated macrophages, controls inflammation by suppressing TNF expression. Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation. {ECO:0000269PubMed:15917799, ECO:0000269PubMed:18172933, ECO:0000269PubMed:20412057, ECO:0000269PubMed:20639877, ECO:0000269PubMed:23583642, ECO:0000269PubMed:23583643, ECO:0000269PubMed:23663784}.

Subcellular Localization

Cytoplasm, P-body {ECO:0000269PubMed:15917799, ECO:0000269PubMed:20412057, ECO:0000269PubMed:20639877, ECO:0000269PubMed:23583642}. Note=During stress, such as that induced by arsenite treatment, localizes to cytosolic stress granules. Localization to stress granules, but not to P-bodies, depends upon the RING-type zinc finger. ICOS repression may correlate with the localization to P- bodies, not to stress granules (PubMed:20639877). {ECO:0000269PubMed:20639877}.

Disease Associations

Tissue Specificity

Widely expressed, with highest levels in lymph node and thymus and slightly lesser amounts in brain, lung, and spleen (at protein level). Very weak expression in heart, muscle, and kidney (at protein level). Expressed in CD4+ helper T-cells (at protein level). {ECO:0000269PubMed:15917799, ECO:0000269PubMed:23583643}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 18 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	18 [view]
Protein-Protein	13 [view]
Protein-DNA	0
Protein-RNA	5 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003729	mRNA binding
GO:0003730	mRNA 3'-UTR binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0044822	poly(A) RNA binding
GO:0046872	metal ion binding

Biological Process

GO:0000956	nuclear-transcribed mRNA catabolic process
GO:0001782	B cell homeostasis
GO:0002634	regulation of germinal center formation
GO:0002635	negative regulation of germinal center formation
GO:0010468	regulation of gene expression
GO:0010608	posttranscriptional regulation of gene expression
GO:0030889	negative regulation of B cell proliferation
GO:0033962	cytoplasmic mRNA processing body assembly
GO:0042098	T cell proliferation
GO:0043029	T cell homeostasis
GO:0043488	regulation of mRNA stability
GO:0045623	negative regulation of T-helper cell differentiation
GO:0046007	negative regulation of activated T cell proliferation
GO:0048535	lymph node development
GO:0048536	spleen development
GO:0050856	regulation of T cell receptor signaling pathway
GO:0061470	T follicular helper cell differentiation
GO:1901224	positive regulation of NIK/NF-kappaB signaling