InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Cdkal1

Summary

InnateDB Protein

IDBP-539889.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Cdkal1

Protein Name

CDK5 regulatory subunit associated protein 1-like 1

Synonyms

1190005B03Rik; 6620401C13Rik;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000122249

InnateDB Gene

IDBG-141263 (Cdkal1)

Protein Structure

UniProt Annotation

Function

Catalyzes the methylthiolation of N6- threonylcarbamoyladenosine (t(6)A), leading to the formation of 2- methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. {ECO:0000250}.

Subcellular Localization

Endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Note=Is a tail-anchored protein that exploits the TCR40 assisted pathway for insertion into the endoplasmic reticulum. {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in pancreas, liver and skeletal muscle, especially in white muscle fibers. {ECO:0000269PubMed:23048041}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	0
Protein-DNA	0
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	3 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003824	catalytic activity
GO:0016740	transferase activity
GO:0035598	N6-threonylcarbomyladenosine methylthiotransferase activity
GO:0046872	metal ion binding
GO:0051536	iron-sulfur cluster binding
GO:0051539	4 iron, 4 sulfur cluster binding

Biological Process

GO:0009451	RNA modification
GO:0035600	tRNA methylthiolation
GO:0043412	macromolecule modification
GO:1990145	maintenance of translational fidelity

Cellular Component

GO:0005783	endoplasmic reticulum
GO:0005789	endoplasmic reticulum membrane
GO:0005791	rough endoplasmic reticulum
GO:0016021	integral component of membrane

Protein Structure and Domains

PDB ID

MGI:1921765

InterPro

IPR002792 TRAM domain
IPR005839 Methylthiotransferase
IPR006466 MiaB-like tRNA modifying enzyme, archaeal-type
IPR006638 Elongator protein 3/MiaB/NifB
IPR007197 Radical SAM
IPR013848 Methylthiotransferase, N-terminal

PFAM

PF01938
PF04055
PF00919

PRINTS

PIRSF

SMART

SM00729

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q91WE6