InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Nos1

Summary

InnateDB Protein

IDBP-540207.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Nos1

Protein Name

nitric oxide synthase 1, neuronal

Synonyms

2310005C01Rik; bNOS; nNOS; NO; Nos-1; NOS-I;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000120421

InnateDB Gene

IDBG-194926 (Nos1)

Protein Structure

UniProt Annotation

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex. {ECO:0000269PubMed:17293453}.

Subcellular Localization

Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine {ECO:0000250}. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity). {ECO:0000250}.

Disease Associations

Note=In MDX mice (mouse model of dystrophinopathy) the dystrophin complex is disrupted and nNOS is displaced from sarcolemma and accumulates in the cytosol.

Tissue Specificity

Widely expressed in the nervous system: expressed in cerebrum, olfactory bulb, hippocampus, midbrain, cerebellum, pons, medulla oblongata, and spinal cord. Also found in skeletal muscle, where it is localized beneath the sarcolemma of fast twitch muscle fibers, and in spleen, heart, kidney, and liver. N-NOS-1 and N-NOS-2 are found in all parts of the nervous system. NNOS beta and gamma occur in a region-specific manner in the brain and NNOS beta expression is developmentally regulated. NNOS Mu is only found in mature skeletal and cardiac muscles.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 19 experimentally validated interaction(s) in this database.
They are also associated with 16 interaction(s) predicted by orthology.

Experimentally validated
Total	19 [view]
Protein-Protein	19 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	16 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003958	NADPH-hemoprotein reductase activity
GO:0004517	nitric-oxide synthase activity
GO:0005506	iron ion binding
GO:0005515	protein binding
GO:0005516	calmodulin binding
GO:0010181	FMN binding
GO:0016491	oxidoreductase activity
GO:0020037	heme binding
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding

Biological Process

GO:0001666	response to hypoxia
GO:0002028	regulation of sodium ion transport
GO:0006809	nitric oxide biosynthetic process
GO:0006941	striated muscle contraction
GO:0007263	nitric oxide mediated signal transduction
GO:0009408	response to heat
GO:0018119	peptidyl-cysteine S-nitrosylation
GO:0031284	positive regulation of guanylate cyclase activity
GO:0033555	multicellular organismal response to stress
GO:0035066	positive regulation of histone acetylation
GO:0042738	exogenous drug catabolic process
GO:0043267	negative regulation of potassium ion transport
GO:0045776	negative regulation of blood pressure
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045909	positive regulation of vasodilation
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0051346	negative regulation of hydrolase activity
GO:0051612	negative regulation of serotonin uptake
GO:0051926	negative regulation of calcium ion transport
GO:0055114	oxidation-reduction process
GO:0071363	cellular response to growth factor stimulus

Cellular Component

GO:0005739	mitochondrion
GO:0005829	cytosol
GO:0005856	cytoskeleton
GO:0016529	sarcoplasmic reticulum
GO:0042383	sarcolemma
GO:0043197	dendritic spine
GO:0045121	membrane raft
GO:0045202	synapse

Protein Structure and Domains

PDB ID

MGI:97360

InterPro

IPR001094 Flavodoxin
IPR001433 Oxidoreductase FAD/NAD(P)-binding
IPR001478 PDZ domain
IPR001709 Flavoprotein pyridine nucleotide cytochrome reductase
IPR003097 FAD-binding, type 1
IPR004030 Nitric oxide synthase, oxygenase domain
IPR008254 Flavodoxin/nitric oxide synthase
IPR012144 Nitric-oxide synthase, eukaryote
IPR017938 Riboflavin synthase-like beta-barrel
IPR029039 Flavoprotein-like

PFAM

PF00175
PF00595
PF13180
PF00667
PF02898
PF00258

PRINTS

PR00369
PR00371

PIRSF

PIRSF000333

SMART

SM00228

TIGRFAMs

Post-translational Modifications

Modification

Cross-References