InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rapgef3

Summary

InnateDB Protein

IDBP-541394.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rapgef3

Protein Name

Rap guanine nucleotide exchange factor (GEF) 3

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000118148

InnateDB Gene

IDBG-178771 (Rapgef3)

Protein Structure

UniProt Annotation

Function

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho- mediated signaling. Required for the actin rearrangement at cell- cell junctions, such as stress fibers and junctional actin (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 10 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	10 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005085	guanyl-nucleotide exchange factor activity
GO:0008603	cAMP-dependent protein kinase regulator activity
GO:0017034	Rap guanyl-nucleotide exchange factor activity
GO:0030552	cAMP binding

Biological Process

GO:0001525	angiogenesis
GO:0007264	small GTPase mediated signal transduction
GO:0032486	Rap protein signal transduction
GO:0032854	positive regulation of Rap GTPase activity
GO:0035556	intracellular signal transduction
GO:0045766	positive regulation of angiogenesis
GO:0045859	regulation of protein kinase activity
GO:0051056	regulation of small GTPase mediated signal transduction
GO:0051496	positive regulation of stress fiber assembly
GO:0061028	establishment of endothelial barrier
GO:0071320	cellular response to cAMP
GO:2000249	regulation of actin cytoskeleton reorganization

Cellular Component

GO:0005622	intracellular
GO:0005737	cytoplasm
GO:0005952	cAMP-dependent protein kinase complex
GO:0016020	membrane

Protein Structure and Domains

PDB ID

MGI:2441741

InterPro

IPR000591 DEP domain
IPR000595 Cyclic nucleotide-binding domain
IPR000651 Ras-like guanine nucleotide exchange factor, N-terminal
IPR001895 Guanine-nucleotide dissociation stimulator CDC25
IPR002373 cAMP/cGMP-dependent protein kinase
IPR018490 Cyclic nucleotide-binding-like
IPR023578 Ras guanine nucleotide exchange factor, domain
IPR029071 Ubiquitin-related domain

PFAM

PF00610
PF00027
PF00618
PF00617

PRINTS

PR00103

PIRSF

SMART

SM00049
SM00100
SM00229
SM00147

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt