Version 5.4
Homo sapiens Protein: APLF
Summary
InnateDB Protein IDBP-54618.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol APLF
Protein Name aprataxin and PNKP like factor
Synonyms
Species Homo sapiens
Ensembl Protein ENSP00000307004
InnateDB Gene IDBG-54616 (APLF)
Protein Structure
UniProt Annotation
Function Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic- apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. {ECO:0000269PubMed:17353262, ECO:0000269PubMed:17396150}.
Subcellular Localization Nucleus. Cytoplasm, cytosol. Note=Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers.
Disease Associations
Tissue Specificity
Comments
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 19 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.
Experimentally validated
Total 19 [view]
Protein-Protein 19 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 1 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0000166 nucleotide binding
GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
GO:0004520 endodeoxyribonuclease activity
GO:0005515 protein binding
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0000012 single strand break repair
GO:0000737 DNA catabolic process, endonucleolytic
GO:0006302 double-strand break repair
GO:0006974 cellular response to DNA damage stimulus
GO:0045191 regulation of isotype switching
GO:0051106 positive regulation of DNA ligation
GO:0090305 nucleic acid phosphodiester bond hydrolysis
Cellular Component
GO:0005634 nucleus
GO:0005829 cytosol
GO:0035861 site of double-strand break
Protein Structure and Domains
PDB ID
InterPro IPR008984 SMAD/FHA domain
IPR019406 Zinc finger, C2H2, APLF-like
PFAM PF10283
PRINTS
PIRSF
SMART
TIGRFAMs
Post-translational Modifications
Modification
Cross-References
SwissProt Q8IW19
PhosphoSite PhosphoSite-Q8IW19
TrEMBL
UniProt Splice Variant
Entrez Gene 200558
UniGene Hs.735377
RefSeq NP_775816
HUGO HGNC:28724
OMIM 611035
CCDS CCDS1888
HPRD 09848
IMGT
EMBL AC105054 AC127383 AC130709 AK290841 BC041144
GenPept AAH41144 AAY14945 AAY24008 AAY24113 BAF83530

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca