InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TOPBP1

Summary

InnateDB Protein

IDBP-57238.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TOPBP1

Protein Name

topoisomerase (DNA) II binding protein 1

Synonyms

TOP2BP1;

Species

Homo sapiens

Ensembl Protein

ENSP00000260810

InnateDB Gene

IDBG-57236 (TOPBP1)

Protein Structure

UniProt Annotation

Function

Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double- stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1- responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. {ECO:0000269PubMed:10498869, ECO:0000269PubMed:11395493, ECO:0000269PubMed:11714696, ECO:0000269PubMed:12697828, ECO:0000269PubMed:15075294, ECO:0000269PubMed:16530042}.

Subcellular Localization

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Note=Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. {ECO:0000250}.

Disease Associations

Tissue Specificity

Highly expressed in heart, brain, placenta, lung and kidney. {ECO:0000269PubMed:9461304}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 77 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	77 [view]
Protein-Protein	72 [view]
Protein-DNA	5 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0005515	protein binding
GO:0008022	protein C-terminus binding
GO:0042802	identical protein binding

Biological Process

GO:0006259	DNA metabolic process
GO:0006281	DNA repair
GO:0006974	cellular response to DNA damage stimulus
GO:0010212	response to ionizing radiation

Cellular Component

GO:0000794	condensed nuclear chromosome
GO:0000922	spindle pole
GO:0001673	male germ cell nucleus
GO:0005634	nucleus
GO:0005694	chromosome
GO:0005737	cytoplasm
GO:0005815	microtubule organizing center
GO:0005886	plasma membrane
GO:0016605	PML body