InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: OAS1

Summary

InnateDB Protein

IDBP-58233.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

OAS1

Protein Name

2'-5'-oligoadenylate synthetase 1, 40/46kDa

Synonyms

IFI-4; OIAS; OIASI;

Species

Homo sapiens

Ensembl Protein

ENSP00000202917

InnateDB Gene

IDBG-58231 (OAS1)

Protein Structure

UniProt Annotation

Function

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L. {ECO:0000269PubMed:12799444, ECO:0000269PubMed:18931074, ECO:0000269PubMed:19923450, ECO:0000269PubMed:23319625}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:19923450}. Mitochondrion {ECO:0000269PubMed:19923450}. Nucleus {ECO:0000269PubMed:19923450}. Microsome {ECO:0000269PubMed:19923450}. Endoplasmic reticulum {ECO:0000269PubMed:19923450}. Secreted {ECO:0000250}. Note=Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 9 experimentally validated interaction(s) in this database.

Experimentally validated
Total	9 [view]
Protein-Protein	5 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001730	2'-5'-oligoadenylate synthetase activity
GO:0003723	RNA binding
GO:0003725	double-stranded RNA binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008270	zinc ion binding
GO:0016740	transferase activity
GO:0016779	nucleotidyltransferase activity
GO:0046872	metal ion binding

Biological Process

GO:0006006	glucose metabolic process
GO:0006164	purine nucleotide biosynthetic process
GO:0006955	immune response
GO:0009615	response to virus
GO:0019221	cytokine-mediated signaling pathway
GO:0035457	cellular response to interferon-alpha
GO:0042593	glucose homeostasis
GO:0045071	negative regulation of viral genome replication
GO:0045087	innate immune response (InnateDB)
GO:0051259	protein oligomerization
GO:0051607	defense response to virus
GO:0060333	interferon-gamma-mediated signaling pathway
GO:0060337	type I interferon signaling pathway
GO:0060700	regulation of ribonuclease activity

Cellular Component

GO:0005576	extracellular region
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005783	endoplasmic reticulum
GO:0005829	cytosol

Protein Structure and Domains

PDB ID

InterPro

IPR002934 Nucleotidyl transferase domain
IPR006116 2-5-oligoadenylate synthetase, N-terminal
IPR018952 2\'-5\'-oligoadenylate synthetase 1, domain 2/C-terminal

PFAM

PF01909
PF10421

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P00973

PhosphoSite