InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PIF1

Summary

InnateDB Protein

IDBP-590810.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PIF1

Protein Name

PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000452792

InnateDB Gene

IDBG-16729 (PIF1)

Protein Structure

UniProt Annotation

Function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity. {ECO:0000255HAMAP-Rule:MF_03176, ECO:0000269PubMed:16522649, ECO:0000269PubMed:17172855, ECO:0000269PubMed:17827721, ECO:0000269PubMed:18835853, ECO:0000269PubMed:19700773, ECO:0000269PubMed:20524933, ECO:0000269PubMed:23657261}.

Subcellular Localization

Nucleus {ECO:0000255HAMAP-Rule:MF_03176, ECO:0000269PubMed:16522649, ECO:0000269PubMed:17172855, ECO:0000269PubMed:17827721}.Isoform 4: Mitochondrion {ECO:0000255HAMAP- Rule:MF_03176, ECO:0000269PubMed:23275553}.

Disease Associations

Tissue Specificity

Weak ubiquitous expression.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.

Experimentally validated
Total	2 [view]
Protein-Protein	1 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000287	magnesium ion binding
GO:0003678	DNA helicase activity
GO:0004386	helicase activity
GO:0005524	ATP binding
GO:0010521	telomerase inhibitor activity
GO:0017116	single-stranded DNA-dependent ATP-dependent DNA helicase activity
GO:0033682	ATP-dependent 5'-3' DNA/RNA helicase activity
GO:0042162	telomeric DNA binding
GO:0043141	ATP-dependent 5'-3' DNA helicase activity
GO:0051880	G-quadruplex DNA binding

Biological Process

GO:0000002	mitochondrial genome maintenance
GO:0000723	telomere maintenance
GO:0006200	ATP catabolic process
GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0032204	regulation of telomere maintenance
GO:0032211	negative regulation of telomere maintenance via telomerase
GO:0032508	DNA duplex unwinding
GO:0051974	negative regulation of telomerase activity