InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: APAF1

Summary

InnateDB Protein

IDBP-592693.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

APAF1

Protein Name

apoptotic peptidase activating factor 1

Synonyms

APAF-1; CED4;

Species

Homo sapiens

Ensembl Protein

ENSP00000448165

InnateDB Gene

IDBG-52364 (APAF1)

Protein Structure

UniProt Annotation

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. {ECO:0000269PubMed:10393175, ECO:0000269PubMed:12804598}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:12804598}.

Disease Associations

Tissue Specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 37 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	37 [view]
Protein-Protein	37 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008656	cysteine-type endopeptidase activator activity involved in apoptotic process
GO:0042802	identical protein binding
GO:0043531	ADP binding

Biological Process

GO:0001843	neural tube closure
GO:0006915	apoptotic process
GO:0006919	activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0006952	defense response
GO:0007275	multicellular organismal development
GO:0007399	nervous system development
GO:0007420	brain development
GO:0008635	activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
GO:0030900	forebrain development
GO:0042981	regulation of apoptotic process
GO:0043065	positive regulation of apoptotic process
GO:0051402	neuron apoptotic process
GO:0072432	response to G1 DNA damage checkpoint signaling
GO:0097193	intrinsic apoptotic signaling pathway
GO:1902510	regulation of apoptotic DNA fragmentation
GO:2001235	positive regulation of apoptotic signaling pathway

Cellular Component

GO:0005634	nucleus
GO:0005829	cytosol
GO:0043293	apoptosome
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR001315 CARD domain
IPR001680 WD40 repeat
IPR002182 NB-ARC
IPR011029 Death-like domain
IPR017251 Apoptotic protease-activating factor 1
IPR017986 WD40-repeat-containing domain
IPR020472 G-protein beta WD-40 repeat
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00619
PF00400
PF00931

PRINTS

PR00320

PIRSF

PIRSF037646

SMART

SM00114
SM00320

TIGRFAMs

Post-translational Modifications

Modification

Cross-References