InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PSTPIP1

Summary

InnateDB Protein

IDBP-595619.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PSTPIP1

Protein Name

proline-serine-threonine phosphatase interacting protein 1

Synonyms

CD2BP1; CD2BP1L; CD2BP1S; H-PIP; PAPAS; PSTPIP;

Species

Homo sapiens

Ensembl Protein

ENSP00000452746

InnateDB Gene

IDBG-24106 (PSTPIP1)

Protein Structure

UniProt Annotation

Function

Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity). Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils. {ECO:0000250, ECO:0000269PubMed:17964261, ECO:0000269PubMed:18480402, ECO:0000269PubMed:19109554, ECO:0000269PubMed:19584923, ECO:0000269PubMed:9857189}.

Subcellular Localization

Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, lamellipodium {ECO:0000250}. Cell projection, uropodium. Cytoplasm, perinuclear region {ECO:0000250}. Cleavage furrow {ECO:0000250}. Note=During interphase, colocalizes with F- actin in the cortical cytoskeleton, lamellipodia, and stress fibers. In dividing cells, colocalizes with the F-actin rich cytokinetic cleavage furrow. Colocalized with WAS to filamentous structures within the cytoplasm. Colocalized with PTPN12 in the cytoplasm and the perinuclear region. Colocalized with CD2AP and WAS in the actin cytoskeleton. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact (By similarity). In monocytes, forms a branched filamentous network in the cytoplasm. In migrating neutrophils, localizes most strongly to the trailing edge of the uropod where it colocalizes with PIP5K1C. In transfected cells, forms relatively straight filaments radiating out from the nucleus. Colocalizes with MEFV, particularly at the branch point of filaments. Filament formation requires an intact tubulin cytoskeleton. {ECO:0000250}.

Disease Associations

PAPA syndrome (PAPAS) [MIM:604416]: Characterized by autosomal dominant inheritance of early-onset, primarily affecting skin and joint tissues. Recurring inflammatory episodes lead to accumulation of sterile, pyogenic, neutrophil-rich material within the affected joints, ultimately resulting in significant destruction. {ECO:0000269PubMed:11971877, ECO:0000269PubMed:22161697}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Tissue Specificity

Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta. {ECO:0000269PubMed:14595024, ECO:0000269PubMed:9857189}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 51 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	51 [view]
Protein-Protein	51 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003779	actin binding
GO:0003824	catalytic activity
GO:0005515	protein binding
GO:0016491	oxidoreductase activity
GO:0019903	protein phosphatase binding

Biological Process

GO:0006897	endocytosis
GO:0006954	inflammatory response
GO:0007155	cell adhesion
GO:0007165	signal transduction
GO:0035872	nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway
GO:0045087	innate immune response (InnateDB)
GO:0055114	oxidation-reduction process

Cellular Component

GO:0001725	stress fiber
GO:0001931	uropod
GO:0005826	actomyosin contractile ring
GO:0005829	cytosol
GO:0016020	membrane
GO:0030027	lamellipodium
GO:0032154	cleavage furrow
GO:0048471	perinuclear region of cytoplasm