InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: POLR1A

Summary

InnateDB Protein

IDBP-59573.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

POLR1A

Protein Name

polymerase (RNA) I polypeptide A, 194kDa

Synonyms

A190; RPA1; RPA194; RPO1-4; RPO14;

Species

Homo sapiens

Ensembl Protein

ENSP00000263857

InnateDB Gene

IDBG-59571 (POLR1A)

Protein Structure

UniProt Annotation

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus, nucleolus {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 44 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	44 [view]
Protein-Protein	41 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001054	RNA polymerase I activity
GO:0003677	DNA binding
GO:0003899	DNA-directed RNA polymerase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding

Biological Process

GO:0006351	transcription, DNA-templated
GO:0006360	transcription from RNA polymerase I promoter
GO:0006361	transcription initiation from RNA polymerase I promoter
GO:0006362	transcription elongation from RNA polymerase I promoter
GO:0006363	termination of RNA polymerase I transcription
GO:0010467	gene expression

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005730	nucleolus
GO:0005736	DNA-directed RNA polymerase I complex
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

InterPro

IPR000722 RNA polymerase, alpha subunit
IPR006592 RNA polymerase, N-terminal
IPR007066 RNA polymerase Rpb1, domain 3
IPR007080 RNA polymerase Rpb1, domain 1
IPR007081 RNA polymerase Rpb1, domain 5
IPR007083 RNA polymerase Rpb1, domain 4

PFAM

PF00623
PF04983
PF04997
PF04998
PF05000

PRINTS

PIRSF

SMART

SM00663

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

O95602