InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RNF31

Summary

InnateDB Protein

IDBP-598370.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RNF31

Protein Name

ring finger protein 31

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000453574

InnateDB Gene

IDBG-3579 (RNF31)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF- induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types. {ECO:0000269PubMed:17006537, ECO:0000269PubMed:19136968, ECO:0000269PubMed:20005846, ECO:0000269PubMed:21455173, ECO:0000269PubMed:21455180, ECO:0000269PubMed:21455181, ECO:0000269PubMed:22863777, ECO:0000269PubMed:23708998}.

Subcellular Localization

Cytoplasm {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in both normal and transformed breast epithelial cell lines. {ECO:0000269PubMed:15093743}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 83 experimentally validated interaction(s) in this database.
They are also associated with 8 interaction(s) predicted by orthology.

Experimentally validated
Total	83 [view]
Protein-Protein	82 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	8 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0043130	ubiquitin binding

Biological Process

GO:0000209	protein polyubiquitination
GO:0016567	protein ubiquitination
GO:0023035	CD40 signaling pathway
GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0045087	innate immune response (InnateDB)
GO:0050852	T cell receptor signaling pathway
GO:0051092	positive regulation of NF-kappaB transcription factor activity
GO:0097039	protein linear polyubiquitination

Cellular Component

GO:0005737	cytoplasm
GO:0009898	cytoplasmic side of plasma membrane
GO:0035631	CD40 receptor complex
GO:0071797	LUBAC complex

Protein Structure and Domains

PDB ID

InterPro

IPR001876 Zinc finger, RanBP2-type
IPR002867 Zinc finger, C6HC-type
IPR009060 UBA-like
IPR011011 Zinc finger, FYVE/PHD-type
IPR011029 Death-like domain
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote

PFAM

PF00641
PF01485

PRINTS

PIRSF

SMART

SM00547
SM00647
SM00165

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q96EP0