InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RAD9A

Summary

InnateDB Protein

IDBP-60165.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RAD9A

Protein Name

RAD9 homolog A (S. pombe)

Synonyms

RAD9;

Species

Homo sapiens

Ensembl Protein

ENSP00000311360

InnateDB Gene

IDBG-60163 (RAD9A)

Protein Structure

UniProt Annotation

Function

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex. {ECO:0000269PubMed:10713044, ECO:0000269PubMed:21659603}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:12628935}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 51 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	51 [view]
Protein-Protein	48 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0008853	exodeoxyribonuclease III activity
GO:0017124	SH3 domain binding
GO:0019899	enzyme binding
GO:0019901	protein kinase binding
GO:0042826	histone deacetylase binding

Biological Process

GO:0000075	cell cycle checkpoint
GO:0000076	DNA replication checkpoint
GO:0000077	DNA damage checkpoint
GO:0000738	DNA catabolic process, exonucleolytic
GO:0006260	DNA replication
GO:0006281	DNA repair
GO:0006974	cellular response to DNA damage stimulus
GO:0031573	intra-S DNA damage checkpoint
GO:0071479	cellular response to ionizing radiation
GO:0090305	nucleic acid phosphodiester bond hydrolysis
GO:1902231	positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage