InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TRIM25

Summary

InnateDB Protein

IDBP-60225.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TRIM25

Protein Name

tripartite motif containing 25

Synonyms

EFP; RNF147; Z147; ZNF147;

Species

Homo sapiens

Ensembl Protein

ENSP00000323889

InnateDB Gene

IDBG-60223 (TRIM25)

Protein Structure

UniProt Annotation

Function

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. {ECO:0000269PubMed:16352599, ECO:0000269PubMed:17069755, ECO:0000269PubMed:17392790}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:17392790}. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies.

Disease Associations

Tissue Specificity

Ubiquitous. {ECO:0000269PubMed:15130519}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 74 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.

Experimentally validated
Total	74 [view]
Protein-Protein	69 [view]
Protein-DNA	5 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	3 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016881	acid-amino acid ligase activity
GO:0044822	poly(A) RNA binding
GO:0046872	metal ion binding

Biological Process

GO:0016032	viral process
GO:0016567	protein ubiquitination
GO:0032480	negative regulation of type I interferon production
GO:0033280	response to vitamin D
GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043627	response to estrogen
GO:0045087	innate immune response (InnateDB)
GO:0046596	regulation of viral entry into host cell
GO:0046597	negative regulation of viral entry into host cell
GO:0051091	positive regulation of sequence-specific DNA binding transcription factor activity
GO:0051092	positive regulation of NF-kappaB transcription factor activity
GO:0051607	defense response to virus
GO:1902186	regulation of viral release from host cell
GO:1902187	negative regulation of viral release from host cell

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005829	cytosol

Protein Structure and Domains

PDB ID

InterPro

IPR001841 Zinc finger, RING-type
IPR001870 B30.2/SPRY domain
IPR003877 SPRY domain
IPR003879 Butyrophylin-like
IPR006574 SPRY-associated
IPR008985 Concanavalin A-like lectin/glucanases superfamily
IPR018957 Zinc finger, C3HC4 RING-type

PFAM

PF13639
PF14634
PF00622
PF13765
PF00097

PRINTS

PR01407

PIRSF

SMART

SM00184
SM00449
SM00589

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt