InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SRSF1

Summary

InnateDB Protein

IDBP-60605.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SRSF1

Protein Name

serine/arginine-rich splicing factor 1

Synonyms

ASF; SF2; SF2p33; SFRS1; SRp30a;

Species

Homo sapiens

Ensembl Protein

ENSP00000258962

InnateDB Gene

IDBG-60601 (SRSF1)

Protein Structure

UniProt Annotation

Function

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. {ECO:0000269PubMed:8139654}.

Subcellular Localization

Cytoplasm. Nucleus speckle. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 171 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	171 [view]
Protein-Protein	166 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003723	RNA binding
GO:0003729	mRNA binding
GO:0005515	protein binding
GO:0044822	poly(A) RNA binding
GO:0050733	RS domain binding

Biological Process

GO:0000395	mRNA 5'-splice site recognition
GO:0000398	mRNA splicing, via spliceosome
GO:0001701	in utero embryonic development
GO:0006366	transcription from RNA polymerase II promoter
GO:0006369	termination of RNA polymerase II transcription
GO:0006376	mRNA splice site selection
GO:0006397	mRNA processing
GO:0006406	mRNA export from nucleus
GO:0008380	RNA splicing
GO:0010467	gene expression
GO:0031124	mRNA 3'-end processing
GO:0060048	cardiac muscle contraction

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0016607	nuclear speck
GO:0035145	exon-exon junction complex
GO:0070062	extracellular vesicular exosome
GO:0071013	catalytic step 2 spliceosome